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  DNA Microarrays: Applications and Data Analysis  
  January 12, 2001

Physiology / Biophysics

 
     
  European Molecular Biology Laboratory (EMBL), Heidelberg, Germany
September 29 - October 6, 2001


Preliminary Content and Topics of the Course:

The DNA microarray is a versatile tool for the experimental exploration of genome structure, gene expression patterns, gene function, the biology of cells and whole organisms. Practical work will include:
- Preparation of DNA samples and slides for the printing, spotting, attachment and hybridisation of DNA on microarray slides
- Design and realisation of experiments for analysis by DNA microarrays
- Detection, processing, analysis, display and interpretation of hybridisation data Experimental applications to be covered in the course will include studies of global gene expression programs, inferring gene function using microarrays, genotyping, and measuring changes in gene copy number.

° Production of DNA microarrays
Sample preparation of RNA
Surface attachment of oligonucleotides and cDNA
Spotting of hybridisation arrays:
Hybridisation protocols
° Microarray spotting systems
Droplet spotting system
Pin spotting systems
Other spotting and arraying techniques
° Detection technology and systems for microarrays
Confocal scanning systems
CCD imaging systems
Other detection methods (chemiluminescence, radioactive, mass spectrometry etc.)
° DNA microarray analysis
Acquisition, image processing and analysis of DNA microarrays
Quantitation of hybridization signals
Visualisation and representation of expression data
Interpretation of datasets
Bioinformatic tools and data bases for expression analysis
° Application of DNA microarrays
Functional genome analysis
Differential gene expression
Genotyping and SNP analysis
Diagnostics, mutation detection and other medical applications
 
 
Organized by: W. Ansorge (EMBL, Heidelberg), A. Brazma (EBI, Hinxton), J. Hoheisel (DKFZ, Heidelberg), Frank Holstege (University Utrecht), J. Quackenbush (TIGR, Rockville)
Invited Speakers: Lecturers and Instructors: (preliminary, to be updated)

Preliminary list of teachers and instructors from the host institute:
Wilhelm Ansorge
Vladimir Benes
Martina Muckenthaler
Thomas Preiss
Alexandra Richter
Bertrand Seraphin
Christian Schwager
Juan Valcarcel
Ernst Wabek

Preliminary list of teachers and instructors from other institutes:
Frank Baas (The Netherlands)
Alvis Brazma (Hinxton, UK)
Dolores Cahill (Berlin, Germany)
Jörg Hoheisel (Heidelberg, Germany)
Frank Holstege (Utrecht, The Netherlands)
Hans Lehrach (Berlin, Germany)
Steffen Lenzner (Berlin, Germany)
Stephen G. Oliver (Manchester, UK)
John Quackenbush (Rockville, USA)
Allan Robinson (Hinxton, UK)
Magnus v. Knebel-Döberitz (Heidelberg, Germany)
Martin Vingron (Heidelberg, Germany)
 
Deadline for Abstracts: August 15, 2001
 
Registration: Application:
The number of participants is limited to 16.
The registration fee is EUR 100,- but may be waived under special circumstances.
Lodging and meals during the course are paid by EMBO.
The applications should contain a short CV, a statement about current research and recent publications.

Application and registration must be submitted ONLINE
E-mail: courses@embl-heidelberg.de
 
  Posted by:   M. Kawaguchi  
Host: mac-photo3.EMBL-Heidelberg.DE
   
 
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