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| HUM-MOLGEN -> Events -> Courses and Workshops | ||||||||||||||||
| Structure Determination of Biological Macromolecules by Solution NMR | ||||||||||||||||
| January 12, 2001 | ||||||||||||||||
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European Molecular Biology Laboratory (EMBL), Heidelberg, Germany September 12-19, 2001 Theme: NMR has established itself as the second method for structure determination of biological macromolecules and is unique in extracting dynamical information on biological macromolecules over a large range of timescales. The intention of the course is to provide practical training in current state-of-the-art NMR techniques and computational methods for structure determination by NMR. The emphasis will be on practical aspects, i.e. setting up experiments on the spectrometer (optimizing parameters, pulse programs), processing and analyzing NMR data, and structure calculations. The course is aimed at PhD students and postdocs who have some working knowledge of NMR theory, i.e. product operator formalism, and who understand the basic principles of NMR pulse sequences. Possible candidates should have the level of knowledge of students that have participated in a past "Advanced NMR" EMBO lecture course on NMR theory organized by Kaptein, Oschkinat and Griesinger. Programme: The course is intended to cover the experimental and computational aspects of characterizing structure and dynamics of a biological macromolecules (proteins and nucleic acids) by modern NMR techniques. This includes setting up and understanding NMR experiments at the spectrometer, triple resonance experiments, edited/filtered experiments, projection angle restraints from residual dipolar couplings and from cross-correlated relaxation, hydrogen bonding, heteronuclear relaxation. Software and methods for processing and analyzing NMR data will be introduced. Structure calculations will be performed using DYANA and ARIA. Every subject will be introduced by lectures (1h each day). The experiments and programs used during the practical will be demonstrated. The remainder of the day will be devoted to practical work either on the spectrometer, or on computers for analyzing NMR data and performing structure calculations. The NMR experiments will be organized in such a way that each participant (in groups of 2-3) will be able to set up the relevant experiments during the week Data analysis, assignment and structure calculation will be performed by the participants in small groups distributed throughout the week. A sample project will be available for performing the NMR experiments, data analysis, assignment and structure calculation. For each step, pre-recorded and pre-processed data will be available. We will try to make it possible for participants to bring their own data. |
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| Organized by: | Michael Nilges, Michael Sattler | |||||||||||||||
| Invited Speakers: | Speakers/ Instructors: Christian Griesinger, Frankfurt Stephan Grzesiek, Basel Peter Guentert, Zuerich Ernest Laue, Cambridge Maria Macias, EMBL Heidelberg Lawrence McIntosh, Vancouver Michael Nilges, EMBL Heidelberg Michael Sattler, EMBL Heidelberg Juergen Schleucher, Umea Harald Schwalbe, Boston Gert Vriend, Nijmegen Geerten Vuister, Nijmegen |
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| Deadline for Abstracts: | July 1, 2001 | |||||||||||||||
| Registration: | The course is planned for a maximum of 20 students. Possible candidates should have the level of knowledge of students that have participated in a past "Advanced NMR" EMBO lecture course on NMR theory organized by Kaptein, Oschkinat and Griesinger. ON-LINE REGISTRATION ONLY |
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| E-mail: | courses@embl-heidelberg.de | |||||||||||||||
| Posted by: | M. Kawaguchi | |||||||||||||||
| Host: | mac-photo3.EMBL-Heidelberg.DE | |||||||||||||||
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