PHAGE DISPLAY OF COMBINATORIAL ANTIBODY LIBRARIES
	Cold Spring Harbor Laboratory , Cold Spring Harbor, NY November 7 - 20, 2000
	Recent advances in the generation and selection of antibodies from combinatorial libraries allow for the rapid production of antibodies from immune and non-immune sources. This intensive laboratory/lecture course will focus on the construction of combinatorial antibody libraries expressed on the surface of phage and selection of desired antibodies from the library. Students will learn the theoretical and practical aspects of constructing combinatorial libraries from immune and non-immune sources as well as the construction of synthetic antibody libraries. Antibodies will be selected from the library by panning. Production, purification and characterization of Fab fragments expressed in E.coli will also be covered. The lecture series, presented by a number of invited speakers, will emphasize PCR of immunoglobulin genes, the biology of filamentous phage and the utility of surface expression libraries, expression of antibodies in E.coli and mammalian cells, antibody structure and function, catalytic antibodies, directed protein evolution, retroviral and cell display libraries, the immunobiology of antibody activity, and recent results on the use of antibodies in therapy. The theory and practical implications for selection from phage displayed libraries of random peptides, cDNA products and semi-synthetic proteins will also be explored.
	Organized by:	Carlos Barbas, Gregg Silverman & Don Siegel
	Deadline for Abstracts:	Application Deadline July 15th
	Registration:	Please register online Deadline July 15th
	Email for Requests and Registration:	Meetings@cshl.org
	Posted by: Michael Glaessgen   Host: talon.cshl.org date: March 09, 2000 17:34:00 Generated by meetings and positions 2.0 by Kai Garlipp Logo and Logo Images by Art for BioMed / Syrinx GmbH - Frankfurt / Germany. 4.1 (c) 1995-99 HUM-MOLGEN. All rights reserved. Liability and Copyright.