Immunocytochemistry, In Situ Hybridization & Live Cell Imaging
	Cold Spring Harbor Laboratory , Cold Spring Harbor, NY October 18 - 31
	Colleague: This course focuses on specialized techniques in microscopy related to localizing DNA, RNA, and proteins in fixed mammalian cells as well as protein dynamics in living cells. The course emphasizes the use of the latest equipment and techniques in epifluorescence microscopy, confocal laser scanning microscopy, digital image processing, and live-cell imaging. The aims of the course were designed to provide state-of-the-art technology and scientific expertise in the use of microscopic applications to address basic questions in cellular and molecular biology. The course is designed for the molecular biologist who is in need of microscopic approaches and for the cell biologist who is not familiar with the practical application of the advanced techniques presented in the course. Among the methods presented are the preparation of tagged nucleic acid probes, fixation methods, detection of multiple DNA sequences in single nuclei or chromosome spreads, comparative genomic hybridization, cellular localization of RNA, localization of nucleic acids and proteins in the same cells, use of a variety of reporter molecules and non-antibody fluorescent tags, indirect antibody labeling, detection of multiple proteins in a single cell, and the use of GFP variants to study protein localization and dynamics. In each method, several experimental protocols are presented allowing the students to assess the relative merits of each and to relate them to their own research. Students are encouraged to bring nucleic acid or antibody probes to the course which are used in addition to those provided by the instructors. The laboratory portion of the course is supplemented by invited lecturers who present up-to-the-minute reports on current methods and research using the techniques being presented in the course.
	Organized by:	John Murray, Thomas Ried & David L. Spector
	Deadline for Abstracts:	Application Deadline July 15th
	Registration:	Please register online Deadline July 15th
	Email for Requests and Registration:	Meetings@cshl.org
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