Imaging Structure and Function in the Nervous System
	Cold Spring Harbor Laboratory , Cold Spring Harbor, NY July 25 - August 14
	Dear Colleague: Advances in light microscopy, digital image processing, and the development of a variety of powerful fluorescent probes present expanding opportunities for investigating the nervous system, from synaptic spines to networks in the brain. This intensive laboratory and lecture course will provide participants with the theoretical and practical knowledge to utilize emerging imaging technologies. The primary emphasis of the course will be on vital light microscopy. Students will learn the principles of light microscopy, as well as use of different types of electronic cameras, laser scanning systems, functional fluorophores, delivery techniques, and digital image-processing software. In addition to transmitted light microscopy for viewing cellular motility, the course will examine a variety of molecular probes of cell function, including calcium-sensitive dyes, voltage-sensitive dyes, photo-activated ("caged") compounds, and exocytosis tracers. Issues arising in the combination of imaging with electrophysiological methods will be covered. Particular weight will be given to multi-photon laser scanning microscopy and to newly available biological fluorophores, especially Green-Fluorescent Protein (GFP) and its variants. We will use a spectrum of neural and cell biological systems, including living animals, brain slices, and cultured cells. Applicants should have a strong background in the neurosciences or in cell biology.
	Organized by:	Kerry Delaney, Venkatesh Murthy & Karel Svoboda
	Invited Speakers:	Lecturers in the 1999 course included: Chiye Aoki, William Betz, Tobias Bonhoeffer, Andrea Brand, John Connor, Richard Day, Paul Forscher, Fritjof Helmchen, Daniel Jay, Rex Kerr, David Kleinfeld, Jeff Lichtman, Greg Macquire, Andrew Matus, Christina Rapp, Williams Schafer, Karel Svoboda, Lansing D. Taylor, Roger Tsien, Clare Waterman-Store, and Shimon Weiss.
	Deadline for Abstracts:	Application Deadline: March 15, 2000
	Registration:	Application Deadline: March 15, 2000 apply online
	Email for Requests and Registration:	meetings@.cshl.org
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