Eukaryotic Gene Expression
	Cold Spring Harbor Laboratory , Cold Spring Harbor, NY July 25 - August 14
	Dear Colleague: The Eukaryotic Gene Expression Course is designed for students, postdocs, and principal investigators who have recently ventured into the dynamic area of gene regulation. The course will focus on state-of-the-art strategies and techniques employed in the field. Cell-free extracts for in vitro transcription will be prepared and RNA levels measured by primer extension analysis. An emphasis will be placed on biochemical studies of protein-DNA and protein-protein interactions. A detailed characterization of the DNA-binding properties of a site-specific transcription factor will be carried out using electrophoretic mobility shift and DNase I foot printing assays. These assays will be used to study protein-DNA interactions in crude extracts and using recombinant proteins purified in class. Both affinity chromatography and co-immunoprecipitation methods will be employed to investigate protein-protein interactions with the general transcription machinery. Over the past few years, the gene regulation field has begun to emphasize the importance of in vivo approaches to studying protein-DNA and protein-protein interactions. Students will therefore be exposed to in vivo footprinting, mapping of DNAse 1 hypersensitive sites, and the yeast two-hybrid methodologies. Experience with basic recombinant DNA techniques is a prerequisite for admission to this course. Lectures by the instructors will cover the current status of the gene expression field, theoretical aspects of the methodology, and broader issues regarding strategies for investigating the regulation of gene expression in eukaryotes. Guest lecturers will discuss contemporary problems in eukaryotic gene regulation and technical approaches to their solution.
	Organized by:	Brian Dynlacht, Grace Gill, James Goodrich & Jacqueline Lees
	Invited Speakers:	The speakers last year included T. Archer, D. Bentley, K. Blackwell, S. Burley, M. Carey, L. Freedman, N. Hernandez, D. Price, J. Reese, S. Smale, J. Workman, R. Young and K. Zaret.
	Deadline for Abstracts:	Application Deadline: March 15, 2000
	Registration:	Application Deadline: March 15, 2000 apply online
	Email for Requests and Registration:	meetings@.cshl.org
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