Molecular Cloning of Neural Genes
	Cold Spring Harbor Laboratory , Cold Spring Harbor, NY June 30 - July 20
	This intensive laboratory and lecture course is intended to teach neuroscientists current approaches to molecular neurobiology. The course consist of daily lectures and laboratory exercises on the practice of molecular neurobiology, with an emphasis on modern approaches to cloning and analyzing the expression of neural genes. A series of evening research seminars by invited speakers focuses on the ways in which these molecular techniques have been successfully applied. In the past, evening seminar topics have included expression cloning, single cell cloning, subtractive cDNA cloning strategies, and genetic and mechanistic studies of neurologic disease, aquisition of cell identity and axon guidance in the developing nervous system. The laboratory portion of the course begins with instruction in a series of basic molecular biological techniques and rapidly advances to more sophisticated methodologies. Students learn to prepare genomic, phage and plasmid DNAs, total and poly (A)+ RNA, and to generate and screen cDNA libraries. Additional topics and methods covered include restriction mapping, agarose and polyacrylamide gel electrophoresis, Northern and Southern blotting, subcloning, oligonucleotide primer design, a selection of PCR-based techniques and the use of nucleotide and protein sequence databases. Gene expression studies include the production of fusion proteins in bacteria and mammalian cell transfection. The advanced techniques feature the construction of cDNA libraries from single cells and the use of subtractive cDNA methods to clone genes expressed in limited populations of cells.
	Organized by:	James Boulter, Catherine Dulac, Cary Lai & Daniel Lavery
	Deadline for Abstracts:	Application Deadline: March 15, 2000
	Registration:	Application Deadline: March 15, 2000 apply online
	Email for Requests and Registration:	meetings@.cshl.org
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