HUM-MOLGEN archive: BIOT: for April

New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 5500 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues! - Please send high quality messages only, including full name, address and purpose. - Please use the appropriate TOPIC subject heading in your message. - Please state non-trivial questions only. - Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community. - Messages may be refused without further notification. Edward Wilcox Trevor M. D'Souza (BIOT editors) **************************************************************** This BIOT message contains: [1] A request for help in releasing Beta-lactamase from the periplasmic space [2] A need for hints in constructing a gene synthetically [3] Does anyone know if the black tailed prairie dog has been karyotyped with banded chromosomes? **************************************************************** [#1] Dear Sir, I work on the detection of any plasmids encoding ampicillin resistance in E. coli and would like to test for beta-lactamase enzyme that is NOT secreted extracellularly. Are there any methods for releasing this enzyme from the periplasmic space? Thanks Sincerely Kuntaman, MD Microbiology Department, Medical Faculty Airlangga University E-mail: kuntaman@mitra.net.id **************************************************************** [#2] Hi all, I am planning a gene synthesis experiment. QUESTIONS: 1) Is there new knowledge on this topic, a new powerful protocol? Otherwise I would like to assemble the gene from overlapping oligonucleotides covering both strands completely, maybe followed by PCR-amplification... any suggestions? 2) Is there a public domain software which helps to split down the DNA sequence to the level of oligonucleotides, helping in avoiding self-complementary oligonucleotides and checking for repeats or other homologous regions that could interfere with correct assembly of the gene? Thanking you in advance - best regards Ralf Koebnik E-mail: koebnik@iname.com **************************************************************** [#3] Does anyone know if the black-tailed prairie dog, Cynomys ludovicianus, has been karyotyped with banded chromosomes? The only report I have found in the literature is pre-banding. Hope Punnett Organization: St. Christopher's Hospital for Children E-mail: punnett@auhs.edu **************************************************************** ************************************************************************ Dr. Arthur A.B. Bergen Department of Ophthalmogenetics The Netherlands Ophthalmic Research Institute (IOI) Royal Academy of Sciences of the Netherlands (KNAW) ** Snail-mail: ** ** FAX: ** ** E-mail: ** P.O.Box 12141 (+31)206916521 A.Bergen@IOI.KNAW.NL 1100 AC Amsterdam The Netherlands ************************************************************************