HUM-MOLGEN archive: (Fwd) BIOT for February

HUM-MOLGEN New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 5500 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues! - Please send high quality messages only, including full name, address and purpose. - Please use the appropriate TOPIC subject heading in your message. - Please state non-trivial questions only. - Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community. - Messages may be refused without further notification. Edward Wilcox Trevor M. D'Souza (BIOT editors) **************************************************************** This BIOT message contains: [1] A request for inhibitors of the PTCH pathway. [2] Are there any liposomes which will not fuse with eukaryotic cells? [3] A request for help in understanding PCR and linkage analysis with microsatellites. [4] A desire to quantify, in ng quantities of human genomic DNA, global DNA methylation levels. [5] A catalogue of Imprinted genes and Parent of Origin Effects is now available. **************************************************************** [#1] I am looking for the following compounds to be used as inhibitors of the PTCH pathway. I appreciate any information on the availability (company or individuals producing them) of these molecules: jervine cyclopamine tomatidine AY9944 triparanol Please send replies to: Dr. Elena Tassi NIH/OPCB/NIDCR, Bldg. 30, Rm. B02 9000 Rockville Pike Bethesda, MD 20892 USA Ph. (301) 496-6233 Fax (301) 402-0823 email: etassi@dir.nidcr.nih.gov **************************************************************** [#2] Hi, I would like to know if there are any liposomes whose constitution can make it not fuse with the membrane of eukaryotic cells. Any information is appreciated. Xiaoli Chen, Xiaoli@hawaii.edu **************************************************************** [#3] Hola!! My name is Andrea Vasconsuelo I would like to know if someone has a protocol for SSCP. I would greatly appreciate receiving some information (protocols, guides, etc.) about PCR and linkage analysis with microsatellites, as well as disease genes, genotyping with microsatellites, and/or any other reprints on the same or related subjects. I work with chromosome 19. Owing to reasons of finance, we are unable to obtain this information by other means. Thank you for your courtesy and look forward to hearing from you soon . Andrea Vasconsuelo <avascon@CRIBA.EDU.AR> **************************************************************** [#4] I wish to quantify, in ng quantities of human genomic DNA, global DNA methylation levels. We're particularly interested in using a mass spec. (preferably) or HPLC method. I would be grateful for any protocols or advice given. Thankyou Elizabeth Hoffman Wessex Human Genetics Institute Southampton General Hospital E.G.Hoffman@SOTON.AC.UK **************************************************************** [#5] I am pleased to advise subscribers that our Catalogue of Imprinted genes and Parent of Origin Effects is now available as a searchable on-line database. The catalogue is based on our recent publication in Human Molecular Genetics (Morison IM and Reeve AE. A catalogue of imprinted genes and parent-of-origin effects in humans and animals. Human Molecular Genetics 1998;7(10): 1599-1609). I endeavour to keep the list as up-to-date as possible but would appreciate notification of new reports that I may have missed. I look forward to your feedback. The database is accessible at: http://www.otago.ac.nz/IGC Ian Morison Senior Research Fellow Cancer Genetics Laboratory Department of Biochemistry University of Otago P O Box 56, Dunedin, New Zealand Tel 64 3 479 7868 Fax 64 3 479 7738 Ian Morison <ian.morison@STONEBOW.OTAGO.AC.NZ> **************************************************************** ************************************************************************ Dr. Arthur A.B. Bergen Department of Ophthalmogenetics The Netherlands Ophthalmic Research Institute (IOI) Royal Academy of Sciences of the Netherlands (KNAW) ** Snail-mail: ** ** FAX: ** ** E-mail: ** P.O.Box 12141 (+31)206916521 A.Bergen@IOI.KNAW.NL 1100 AC Amsterdam The Netherlands ************************************************************************