home   genetic news   bioinformatics   biotechnology   literature   journals   ethics   positions   events   sitemap
 
  HUM-MOLGEN -> mail archive   |   Search register for news alert (free)  
  Arthur Bergen: BIOT: for September  
   

archive of HUM-MOLGEN mails

 
 

[Author Prev][Author Next][Thread Prev][Thread Next][Author Index][Topic Index]

To: HUM-MOLGEN@NIC.SURFNET.NL
Subject: BIOT: for September
From: Arthur Bergen <a.bergen@ioi.knaw.nl>
Date: Thu, 15 Oct 1998 11:36:59 MET
Organization: ioi.knaw.nl
Priority: normal

New BIOTs!

The BIOT section is open for requests and offers of information
regarding molecular biology and molecular genetics (protocols,
techniques, products, collaboration, etc.).

You can reach over 5016 of your colleagues, and on average you may
expect up to twenty replies to a single message. This service is
absolutely FREE of charge. Help yourself by helping your colleagues !

- Please send high quality messages only, including full name,
  address and purpose.
- Please use the appropriate TOPIC subject heading in your message.
- Please state non-trivial questions only.
- Please reply by private E-mail only, unless your request is of
  general interest to the entire HUM-MOLGEN community

Messages may be refused without further notification.

Edward Wilcox
Trevor M. D'Souza
(BIOT editors)

=======================================================

This BIOT message contains:

1) Is there a better method than Alkaline Phosphatase direct labeling with
   chemiluminescence detection for DNA probes?
2) Does anyone have a reliable protocol for extraction of PCRable DNA from
   liver needle biopsy?
3) We are interested in obtaining EBV-immortalized lymphoblasts from Seckel
   syndrome patients to be used for cloning the disease gene.
4) Has anyone come across any literature about the presence of mutant p53 in
   normal tissue or body fluids?
5) I am looking for libraries from human testis for either phage display or
   yeast two-hybrid assays.
6) An on-line chat on Trends on Nutraceuticals.
7) An annotated directory of online molecular biology research tools
   (BioToolKit).
8) An electronic copy of Dr. Jianping Qiu's article Protein Expression System
   is available on the web.

******************************************************************************
Dear Sir/Madam:

Is there anyone with experience in using Alkaline Phosphatase direct
labeling with chemiluminescence detection? I would like to use Alkaline
Phosphatase labeling on an oligonucleotide probe (~ 30 mers). There are two
kits we are considering (Amersham and Boehringer). Any suggestions would be
appreciated or please share your experiences with us.

Sincerely,
Pornprot Limprasert M.D., Ph.D.
Human Genetics Unit
Department of Pathology
Faculty of Medicine
Prince of Songkla University
Had Yai, Songkhla 90110
Thailand
Tel 66-74-2129070 to 9 ext. 1584
Fax 66-74-212908, 212903
E-mail: Lpornpro@ratree.psu.ac.th
******************************************************************************
Dear all,

We have a liver needle biopsy that has been stained with ?? (H & E). It is vital
to extract DNA from this to offer a diagnosis to a family.

Does anyone have a reliable protocol for extraction of PCRable DNA from
something like this?

All input appreciated.

Yours
Dr Hamish S. Scott
Division of Medical Genetics
University of Geneva Medical School
1 rue Michel Servet
1211 Geneva 4
SWITZERLAND

Ph:  (+41 22) 702 5719
Fax: (+41 22) 702 5706
E-mail: Hamish.Scott@medecine.unige.ch
******************************************************************************
Dear colleagues,

Our lab is involved in the study of Fanconi anemia, a rare chromosomal
instability disorder with hypersensitivity to crosslinking agents. We have used
somatic cell hybridization to demonstrate the existence of 8 complementation
groups, each of which is thought to be connected to a distinct FA disease gene.
Two of these have now been cloned by complementation cloning, and a third is
underway.

Seckel syndrome is a rare disease, also characterized by hypersensitivity to
crosslinking agents. We are interested in obtaining EBV-immortalized
lymphoblasts from Seckel syndrome patients to be used for cloning the disease
gene by complementation cloning.

If you have such lymphoblasts in your collection and if you are interested in a
collaboration on cloning the Seckel syndrome gene, please get in touch.

Dr. H. Joenje
Department of Human Genetics
Free University
Van der Boechorststraat 7
NL-1081 BT Amsterdam
The Netherlands
Tel +31-20-4448270
Fax +31-20-4448285
E-mail: H.Joenje.HumGen@med.vu.nl
******************************************************************************
I am a research assistant working on mutant p53 in oral cavity cancers. I
wonder if anyone has any literature about the presence of mutant p53 in normal
tissue or body fluids.(Yes, in some cases it has been detected.).

Please contact me via my E-mail  addresses.

Pelin GUERDAL

E-mail:
guerdal@dishekimligi.ege.edu.tr
agurdal@superonline.com
******************************************************************************
Hi,

I am interested in looking for interacting proteins in sperm-egg binding. I am
looking for libraries from human testis for either phage display or yeast
two-hybrid assays. If anyone has either of the above they are willing to
share, then I would be very interested. Alternatively, if anyone has experience
of using a commercial library, then I would be interested in their comments.

Thank you for your time.

David C. Hughes

Assisted Conception Unit,
Birmingham Womens Hospital
UK

E-mail: dhughes@hgmp.mrc.ac.uk
******************************************************************************
On October 22nd, 13:30, ET (Montreal), we are organizing an on-line chat at
http://www.foodonline.com/chat/ on Trends on Nutraceuticals.  Professionals
from academia, food and pharmaceutical industry, NGO, financial institutions,
governmental organizations are being invited.

Join our meeting that will include aspects related to processing, market
potential, and new trends on prebiotics, probiotics, phytochemicals,
oligoelements, and food products.

Looking forward to meeting you,
Marcio Belem, Ph. D.
Belem Biotech
http://www.geocities.com/Eureka/Park/9073
E-mail: bel_ab@msn.com
******************************************************************************
Dear Colleagues:

Following is the Publication News from Syncomm Corp.:

Dr. Jianping Qiu's article Protein Expression System has been presented in the
August Issue of Genetic Engineering News, giving an overview of the current
trends in systems for protein expression. Anyone who might be interested in
recombinant protein engineering and expression may request an electronic copy
of this article at linderfei@syncommbdl.com. You are also welcome to discuss
any protein expression issues with Dr.Jianping Qiu at jpq@syncommbdl.com

Any request or inquiry about the biotech services are also welcome:

1. Recombinant protein (including human recombinant antibodies) engineering
and expression
2. Recombinant protein production
3. Custom construction and biopanning of phage display system.
4. In vitro mutagenesis (see more at http://www.syncommbdl.com)

Thanks and regards.

Linda Fei
Marketing Manager
Syncomm Corporation
3401 Market Street
Philadelphia, PA 19104, USA
T. 215.387.2811
F. 215.387.2886
E-mail: lindafei@syncommbdl.com
******************************************************************************
The BioToolKit (http://www.biosupplynet.com/cfdocs/btk/btk.cfm ), an annotated
directory of online molecular biology research tools, lists 710 free resources
including 32 molecular biology protocols. This directory, which was updated a
week ago, is unique in emphasizing biomacromolecular structural display tools
and the structural comparison programs that utilize these tools.

Cynthia S. Smagula, Ph.D.
BIOTA Publications
4700 Ridgedale Drive
Plano, TX 75024
972.618.3022
E-mail: biota@ONRAMP.NET
******************************************************************************
************************************************************************
Dr. Arthur A.B. Bergen
Department of Ophthalmogenetics
The Netherlands Ophthalmic Research Institute (IOI)
Royal Academy of Sciences of the Netherlands (KNAW)

** Snail-mail: **           ** FAX: **             ** E-mail: **

P.O.Box 12141               (+31)206916521         A.Bergen@IOI.KNAW.NL
1100 AC  Amsterdam
The Netherlands
************************************************************************


   
 
home   genetic news   bioinformatics   biotechnology   literature   journals   ethics   positions   events   sitemap
 
 
 

Mail converted by MHonArc 2.4.4
WWW: Kai Garlipp, Frank S. Zollmann.
7.0 1995-2001 HUM-MOLGEN. All rights reserved. Liability and Copyright.