HUM-MOLGEN archive: BIOT: for September

New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 5016 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues ! - Please send high quality messages only, including full name, address and purpose. - Please use the appropriate TOPIC subject heading in your message. - Please state non-trivial questions only. - Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community Messages may be refused without further notification. Edward Wilcox Trevor M. D'Souza (BIOT editors) ======================================================= This BIOT message contains: 1) Is there a better method than Alkaline Phosphatase direct labeling with chemiluminescence detection for DNA probes? 2) Does anyone have a reliable protocol for extraction of PCRable DNA from liver needle biopsy? 3) We are interested in obtaining EBV-immortalized lymphoblasts from Seckel syndrome patients to be used for cloning the disease gene. 4) Has anyone come across any literature about the presence of mutant p53 in normal tissue or body fluids? 5) I am looking for libraries from human testis for either phage display or yeast two-hybrid assays. 6) An on-line chat on Trends on Nutraceuticals. 7) An annotated directory of online molecular biology research tools (BioToolKit). 8) An electronic copy of Dr. Jianping Qiu's article Protein Expression System is available on the web. ****************************************************************************** Dear Sir/Madam: Is there anyone with experience in using Alkaline Phosphatase direct labeling with chemiluminescence detection? I would like to use Alkaline Phosphatase labeling on an oligonucleotide probe (~ 30 mers). There are two kits we are considering (Amersham and Boehringer). Any suggestions would be appreciated or please share your experiences with us. Sincerely, Pornprot Limprasert M.D., Ph.D. Human Genetics Unit Department of Pathology Faculty of Medicine Prince of Songkla University Had Yai, Songkhla 90110 Thailand Tel 66-74-2129070 to 9 ext. 1584 Fax 66-74-212908, 212903 E-mail: Lpornpro@ratree.psu.ac.th ****************************************************************************** Dear all, We have a liver needle biopsy that has been stained with ?? (H & E). It is vital to extract DNA from this to offer a diagnosis to a family. Does anyone have a reliable protocol for extraction of PCRable DNA from something like this? All input appreciated. Yours Dr Hamish S. Scott Division of Medical Genetics University of Geneva Medical School 1 rue Michel Servet 1211 Geneva 4 SWITZERLAND Ph: (+41 22) 702 5719 Fax: (+41 22) 702 5706 E-mail: Hamish.Scott@medecine.unige.ch ****************************************************************************** Dear colleagues, Our lab is involved in the study of Fanconi anemia, a rare chromosomal instability disorder with hypersensitivity to crosslinking agents. We have used somatic cell hybridization to demonstrate the existence of 8 complementation groups, each of which is thought to be connected to a distinct FA disease gene. Two of these have now been cloned by complementation cloning, and a third is underway. Seckel syndrome is a rare disease, also characterized by hypersensitivity to crosslinking agents. We are interested in obtaining EBV-immortalized lymphoblasts from Seckel syndrome patients to be used for cloning the disease gene by complementation cloning. If you have such lymphoblasts in your collection and if you are interested in a collaboration on cloning the Seckel syndrome gene, please get in touch. Dr. H. Joenje Department of Human Genetics Free University Van der Boechorststraat 7 NL-1081 BT Amsterdam The Netherlands Tel +31-20-4448270 Fax +31-20-4448285 E-mail: H.Joenje.HumGen@med.vu.nl ****************************************************************************** I am a research assistant working on mutant p53 in oral cavity cancers. I wonder if anyone has any literature about the presence of mutant p53 in normal tissue or body fluids.(Yes, in some cases it has been detected.). Please contact me via my E-mail addresses. Pelin GUERDAL E-mail: guerdal@dishekimligi.ege.edu.tr agurdal@superonline.com ****************************************************************************** Hi, I am interested in looking for interacting proteins in sperm-egg binding. I am looking for libraries from human testis for either phage display or yeast two-hybrid assays. If anyone has either of the above they are willing to share, then I would be very interested. Alternatively, if anyone has experience of using a commercial library, then I would be interested in their comments. Thank you for your time. David C. Hughes Assisted Conception Unit, Birmingham Womens Hospital UK E-mail: dhughes@hgmp.mrc.ac.uk ****************************************************************************** On October 22nd, 13:30, ET (Montreal), we are organizing an on-line chat at http://www.foodonline.com/chat/ on Trends on Nutraceuticals. Professionals from academia, food and pharmaceutical industry, NGO, financial institutions, governmental organizations are being invited. Join our meeting that will include aspects related to processing, market potential, and new trends on prebiotics, probiotics, phytochemicals, oligoelements, and food products. Looking forward to meeting you, Marcio Belem, Ph. D. Belem Biotech http://www.geocities.com/Eureka/Park/9073 E-mail: bel_ab@msn.com ****************************************************************************** Dear Colleagues: Following is the Publication News from Syncomm Corp.: Dr. Jianping Qiu's article Protein Expression System has been presented in the August Issue of Genetic Engineering News, giving an overview of the current trends in systems for protein expression. Anyone who might be interested in recombinant protein engineering and expression may request an electronic copy of this article at linderfei@syncommbdl.com. You are also welcome to discuss any protein expression issues with Dr.Jianping Qiu at jpq@syncommbdl.com Any request or inquiry about the biotech services are also welcome: 1. Recombinant protein (including human recombinant antibodies) engineering and expression 2. Recombinant protein production 3. Custom construction and biopanning of phage display system. 4. In vitro mutagenesis (see more at http://www.syncommbdl.com) Thanks and regards. Linda Fei Marketing Manager Syncomm Corporation 3401 Market Street Philadelphia, PA 19104, USA T. 215.387.2811 F. 215.387.2886 E-mail: lindafei@syncommbdl.com ****************************************************************************** The BioToolKit (http://www.biosupplynet.com/cfdocs/btk/btk.cfm ), an annotated directory of online molecular biology research tools, lists 710 free resources including 32 molecular biology protocols. This directory, which was updated a week ago, is unique in emphasizing biomacromolecular structural display tools and the structural comparison programs that utilize these tools. Cynthia S. Smagula, Ph.D. BIOTA Publications 4700 Ridgedale Drive Plano, TX 75024 972.618.3022 E-mail: biota@ONRAMP.NET ****************************************************************************** ************************************************************************ Dr. Arthur A.B. Bergen Department of Ophthalmogenetics The Netherlands Ophthalmic Research Institute (IOI) Royal Academy of Sciences of the Netherlands (KNAW) ** Snail-mail: ** ** FAX: ** ** E-mail: ** P.O.Box 12141 (+31)206916521 A.Bergen@IOI.KNAW.NL 1100 AC Amsterdam The Netherlands ************************************************************************