HUM-MOLGEN archive: BIOT: for June

New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 5016 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues! - Please send high quality messages only, including full name, address and purpose. - Please use the appropriate TOPIC subject heading in your message. - Please state non-trivial questions only. - Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community Messages may be refused without further notification. Edward Wilcox Trevor M. D'Souza (BIOT editors) This BIOT message contains: 1) Request for help with touch down PCR 2) Please help find the origin of a sequence. 3) How much is known about MER22 interspersed repeats? ********************************************************************** Dear colleagues: I tried several times to do touch down PCR, but failed and I got a smear. Can anyone help? Hosam SHAMS-ELDIN shamseld@MAILER.UNI-MARBURG.DE -- Zentrum fuer Hygiene AG Parasitologie Robert-Koch-Strasse 17 D-35037 Marburg Tel.:06421/285493 Fax :06421/285482 ********************************************************************** We have recently characterized an exonic insertion of 38-bp in a patient with an X-linked myopathy. We are looking for the origin of this sequence which contains 2 repeated motifs in tandem :ga gatcatatct gtcatat gatcatatct tt gtcatat (38bp). Could anyone help us find the origin of this insertion? Your experience in repeated sequence insertions would be of great help. Sylvie Tuffery (PhD) Laboratoire de Biochimie Genetique Institut de biologie Bd Henri IV 34060 Montpellier cedex - France. Tel : 33 4 67 60 95 06 Fax : 33 4 67 60 11 81 e-mail : tuffery@mail.igh.cnrs.fr ********************************************************************** Does anybody know anything about the MER22 interspersed repeat (mechanism of transposition etc.)? If yes, please contact me. fatykar@sc.llu.edu (Karoly Fatyol) ********************************************************************** ************************************************************************ Dr. Arthur A.B. Bergen Department of Ophthalmogenetics The Netherlands Ophthalmic Research Institute (IOI) Royal Academy of Sciences of the Netherlands (KNAW) ** Snail-mail: ** ** FAX: ** ** E-mail: ** P.O.Box 12141 (+31)206916521 A.Bergen@IOI.KNAW.NL 1100 AC Amsterdam The Netherlands ************************************************************************