HUM-MOLGEN archive: BIOT: December 1997

------- Forwarded Message Follows ------- Date: Thu, 15 Jan 1998 11:09:18 -0500 Reply-to: Human Molecular Genetics Editors <ED-MOLGEN@NIC.SURFNET.NL> From: Edward Wilcox <edwilcox@POP.NIDCD.NIH.GOV> Subject: BIOT for December To: ED-MOLGEN@NIC.SURFNET.NL New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 4250 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues! -Please send high quality messages only, including full name, address and purpose. -Please use the appropriate TOPIC subject heading in your message. -Please state non-trivial questions only. -Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community Messages may be refused without further notification. Edward Wilcox Arthur Bergen (BIOT editors) This BIOT message contains: 1) An interest in molecular modeling programs. 2) An interest in the background of the HuTu 80 cell line. 3) An interest in how to purify hCG. 4) A question concerning viruses. 5) An interest in the average diameter of allergens. 6) What is the genomic organization of the human TGF-beta 1 gene? ***************************************************************************** 1) An interest in molecular modeling programs I have some problems to solve on protein folding. Does anyone know if there is a molecular modeling program that allows to know how changing an amino acid may affect the folding of the mature protein? Or how the same changed amino acid might affect a dimeric or trimeric structure? Thank you for any suggestion Ludovica Volpi ludovica@imr.gov.my Remote IP address: 202.184.120.39 ***************************************************************************** 2) An interest in the background of the HuTu 80 cell line. The HuTu 80 cell line was established from a duodenal adenocarcinoma in a 53 yr. old male. Does anyone know if that person had Familial Adenomatous Polyposis or alternatively has anyone found an APC mutation in these cells? Thanks Joanne Young PhD Senior Scientist Conjoint Gastroenterology Laboratory Bancroft Centre, 300 Herston Road, Herston Q4029 Australia joanney@qimr.edu.au phone: 61 7 3362 0490 fax: 61 7 3362 0108 **************************************************************************** 3) An interest in how to purify hCG Dear Colleague, We want to purify hCG from the urine of pregnant females, but our first attempt at chromatography yielded very little product. Can we use electrophoresis for separation of alpha & beta hCG? Please reply us as soon as possible! With my best regards, Happy Holiday, Sincerely Yours Firouzeh Biramijamal Department of Biochemistry Faculty of Medical Sciences Tarbiat Modarres University P.O.Box 14155-4838 Tehran I.R.IRAN e-mail beyrami@nrcgeb.as.ir ***************************************************************************** 4) A question concerning viruses. I am a high school student with a question concerning viruses. Please correct me if I am wrong in anything I say. I am speaking of the lysogenic cycle. A virus enters the cell and becomes a provirus usually. Because the virus would be destroyed if a lytic virus entered the cell it would make sense for the lysogenic virus to either destroy other viruses or keep them out of the cell. It would also be a waste for other identical viral particles to infect the cell. I also realize that many animal viruses have a membranous envelope from the cell onto which they have placed their own glycoproteins for recognition purposes. When the virus enters it leaves some glycoproteins on the cell membrane and it adds some before it exits. Taking this into consideration, I wondered if viruses have the ability to change the outside of the cell so that other viruses could not recognize the cell while maintaining what is necessary for the cell to function. Justin ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: Remote IP address: 32.96.49.180 olmbickf@bellsouth.net ***************************************************************************** 5) An interest in the average diameter of allergens. Does anyone know of a good literary source that lists the average diameter of allergens associated with common household animals? I am working on a masters' thesis dealing with indoor air pollution abatement systems. I need the average sizes of the allergens(e.g., Fel d I, etc.) from their associated sources, i.e., cats, dogs, etc. My background is in chemical engineering and we often refer to C.E. Lapple's well known "Size and Characteristics of Airborne Particles" chart. Unfortunately, none of the allergens are listed. Thank you very much! Malcolm Windsor mwindsor@futura.net ***************************************************************************** 6) What is the genomic organization of the human TGF-beta 1 gene? Could anyone out there supply me with data on the genomic organization (including intron sizes) of the human TGF-beta 1 gene? If not, does anyone have a genomic clone containing the TGFB1 gene that they would be willing to send me? Thank you very much for your help. Fernando Gibson, Senior Research Scientist GEMINI RESEARCH LTD 162 Science Park, Milton Road Cambridge, UK CB4 4GH Tel: +44 (0)1223 435320 Fax: +44 (0)1223 435301 Email: fernando.gibson@gemini-research.co.uk ***************************************************************************** ************************************************************************ Dr. Arthur A.B. Bergen Department of Ophthalmogenetics The Netherlands Ophthalmic Research Institute (IOI) Royal Academy of Sciences of the Netherlands (KNAW) ** Snail-mail: ** ** FAX: ** ** E-mail: ** P.O.Box 12141 (+31)206916521 A.Bergen@IOI.KNAW.NL 1100 AC Amsterdam The Netherlands ************************************************************************