HUM-MOLGEN archive: BIOT: January 1998

New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 4250 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues! -Please send high quality messages only, including full name, address and purpose. -Please use the appropriate TOPIC subject heading in your message. -Please state non-trivial questions only. -Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community Messages may be refused without further notification. Edward Wilcox Arthur Bergen (BIOT editors) This BIOT message contains: 1) A search for a cDNA-probe of human preproIGF-II 2) help with the genomic structure of the calcineurin A gene 3) a question regarding immuno histochemistry in plant tissue 4) A request for a CAT or luciferase or green fluorescent cDNA construct that is exclusively under control of the T7 promoter 5) a very basic genetics question on the inheritability of hair color 6) request for a protocol for extracting DNA from methanol/glacial acetic acid fixed cells 7) A search for websites listing chromosomal variants and anomalies 8) A question about making an adenovirus with a gene of interest 9) Requesting information on possible sources of human genomic DNA from unrelated individuals 10) An interest in buying used genetics textbooks 11) An interest in the recent discovery of the gene that causes aging ***************************************************************************** Please post: We are interested in the role of IGF-II in the heart. To elucidate this further we would like to transfect mouse hearts using human IGF-II adenoviral constructs. Does anybody have a cDNA-probe for human preproIGF-II encoding the complete IGF-II precursor protein? Any help would be appreciated. Thanks Dr. Rene Zimmermann Max-Planck-Institute (W.G. Kerckhoff-Institute) & Kerckhoff-Clinic Dept. of Experimental Cardiology Phone: +49/6032/705-404 Benekestrasse 2 Fax: +49/6032/705-419 D-61231 Bad Nauheim e-mail:rzimmer@kerckhoff.mpg.de GERMANY ***************************************************************************** Can anybody provide me with the genomic structure of the calcineurin A gene which maps to human chromosome 4q or with a genomic clone comprising this gene. Your help is greatly appreciated. Please contact: Olaf Riess, MD Molecular Human Genetics Ruhr-University 44780 Bochum Germany phone: +49-2347003831 fax: +49-2347094196 email: olaf.riess@ruhr-uni-bochum.de ***************************************************************************** Dear all, I have a question regarding immuno histochemistry in plant tissue. I am looking for a nuclear antigen; could anyone please suggest a protocol? thanx, sushma nagaraj sushma75@hotmail.com **************************************************************************** We have cells in culture that stably express T7 RNA polymerase. We would like to test this by transfecting a CAT or luciferase or green fluorescent cDNA construct that is exclusively under control of the T7 promoter, i.e. no eukaryotic promoter should be present in this construct. If anyone out there has such a plasmid, I would be very grateful for a reply. Thank you very much in advance. Jasper zu Putlitz Jasper zu Putlitz, M.D. Molecular Hepatology Laboratory MGH Cancer Center Harvard Medical School 149, 13th Street Charlestown, MA 02129 USA Phone: +1-617-724-5561 Fax: +1-617-726-5609 e-mail: jputlitz@helix.mgh.harvard.edu ***************************************************************************** I'm hoping someone can steer me in the right direction regarding a very basic genetics question on the inheritability of hair color. Specifically, I was curious to know if hair color followed a similar pattern to eye color, with darker hair being "dominant" over lighter hair, as brown eyes are dominant over blue. Obviously I'm using the model here of eye color being a simple trait (and I understand it's not quite that straightforward in the real world) wherein parenta with brown eyes can have children with blue eyes (25% of the time), but parents with blue eyes can only produce blue-eyed offspring. My question is: can this admittedly oversimplified model also be applied to hair color, in which two fair-haired parents should only produce blond offspring? If not, under what circumstances could two blond parenta have a decidedly brunette child (for example, if one of the parents' parents had dark hair)? I apologize for injecting this high-school level question into a professional discussion area such as this, but if anyone could help me clarify this issue I'd be most grateful. Thank you. John Bertram, Toronto John_Bertram@TVO.ORG ***************************************************************************** Dear everyone, I wonder if anyone has the protocol for extracting DNA from methanol/glacial acetic acid fixed cells (initially intended for for cytogenetics)? Your help is much apprecaited. Thank you very much. Yours sincerely, C.S.Kua Institute of Health & Community Medicine, Universiti Malaysia sarawak 94300 Kota Samarahan, Sarawak, Malaysia. Fax : 6-082-671903 E-mail : cskua@tualang.unimas.my or cskua@hotmail.com ***************************************************************************** I am looking for the WWW page on Chromosomal variation in Man. A catalog of chromosomal variants and anomalies and The repository/Registratry (Repository of human chromosomal variants and anomalies/international registry of abnormal karyotypes). I would appreciate any information. Thank you Pornprot Limprasert M.D., Ph.D. Human Genetics Unit, Department of Pathology Faculty of Medicine Prince of Songkla University Thailand Lpornpro@ratree.psu.ac.th ***************************************************************************** I have two questions and hope someone can help me find answers. 1. How long is activated PKC activity sustained after the stimulation of EGF? Are there any references with information? 2. To make an adenovirus containing the gene of interest, I need to co-transfect both the "arms" of the adenovirus and the plasmid vector containing the gene into 293 cells and collect the cells containing adenovirus which has the recombinant DNA. This process is time consuming and not gauranteed to get the desired result. I am wondering whether there is a better way, say, let this recombinant process happen in vitro, just like we do the ligation of a DNA fragment with plasmid DNA? Any suggestions will be highly appreciated. Thanks. Huicong Cai NIH 6/214 email: hcai@helix.nih.gov ***************************************************************************** Requesting information on possible sources (commercial, govenmental, etc) of human genomic DNA from unrelated individuals to allow studies of allele frequency in the population. Eric T. Everett, Ph.D. Assistant Professor Oral-Facial Genetics Indiana University School of Dentistry eeverett@iusd.iupui.edu ***************************************************************************** I am interested in buying used genetics textbooks, study guides, problem and solutions books. Please let me know the title and price of the book you may wish to sell. Thanks a lot for your help. milyas@home.com (Mohammad Ilyas) ***************************************************************************** I am a student at Canadore College in North Bay, Ont. Canada. I an doing an essay on biomedical ethics, and am quite interested in the recent discovery of the gene that causes aging. If you can refer me to an address where I might find further news, or any news on this topic at all, I would greatly appreciate it, since I have not had any luck in finding such information on the internet myself. Thank you, Sincerely, Joen Seddon m.jane@efni.com *****************************************************************************