HUM-MOLGEN archive: BIOT: for November

New BIOTs! The BIOT section is open for requests and offers of information regarding molecular biology and molecular genetics (protocols, techniques, products, collaboration, etc.). You can reach over 5016 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues! - Please send high quality messages only, including full name, address and purpose. - Please use the appropriate TOPIC subject heading in your message. - Please state non-trivial questions only. - Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community Messages may be refused without further notification. Edward Wilcox Trevor M. D'Souza (BIOT editors) ------------------------------------------------------------------- This BIOT message contains: 1) Need help on isolation of genomic DNA from slides 2) A request for P19 cell line 3) I need information on competitive PCR 4) Need information about Bio-Informatics 5) Need information regarding genetic studies on Chronic Idiopathic Thrombocytopnenic purpura 6) Where can I find information on the gene/s that contribute to eye color? ------------------------------------------------------------------ [#1] I am trying to extract genomic DNA from lung tissues that were fixed on slides and am having difficulty recovering DNA that can serve as template for PCR amplification. These slides were stained with H&E and were paraffin embedded. Does anyone have experience isolating human genomic DNA from tissues fixed on slides and successfully amplifying it by PCR? Thank you for your help. Nancy C. Arbour, Ph.D. Assistant Research Scientist Department of Internal Medicine University of Iowa 2182 Med Labs Iowa City, IA 52242 E-mail: Nancy-Arbour@MAIL.INT-MED.UIOWA.EDU ------------------------------------------------------------------ [#2] I am a graduate student at the University of Cincinnati and have been having problems with the P19 mouse EC cells that we have been getting from ATCC. The company insists it is not their cell line that is contaminated, but our samples (all from the same lot) seem to be contaminated upon arrival. Does anyone else have this problem? Does anyone have viable P19 cells that we could obtain? We have already lost several weeks to this problem. Any help would be greatly appreciated. Alex Ramirez Dept. of Biological Sciences University of Cincinnati E-mail: ramireae@email.uc.edu ------------------------------------------------------------------ [#3] I am interested in competitive PCR techniques using a plasmid as control substrate for amplification. I have never used such methods and am looking for troubleshooting advice. I am also looking for new methods for quantitative mRNA and cDNA analysis based on PCR. Wojciech Mlynarski, MD, MSc Molecular Biology Unit, Institute of Peadiatrics, Medical University of Lodz Sporna Str 36/50, 91-738 Lodz Poland phone/fax +48 42 6562902 E-mail: mlynek@alef.am.lodz.pl ------------------------------------------------------------------ [#4] How do I locate Bio-Informatics professors and their students? Is there some sort of Bio-Informatics journal? Please advise. Shellie B. Williams E-mail: williams@BIOWEBDIR.COM ------------------------------------------------------------------ [#5] I am a scientific doctor at the Research Institute of Pediatrics in Moscow, Hematology department. My recent theme of studies is Chronic Idiopathic Thrombocytopnenic purpura. I wonder if anyone can provide me with information regarding possible genetic research done in this field, or give me an idea of specific genetic studies I can do. Any advice is highly appreciated. Sincerely, Dr. Victoria Khouloudi, Research Institute of Pediatrics Lomonossovsky pr. 6/2, Tel # 7 095 - 134 15 82 Fax # 7 095 - 280 25 12 E-mail: VKhouloudi@intsos.com ------------------------------------------------------------------ [#6] My AP Biology class and I are trying to find information on the gene/s that contribute to eye color. We understand brown and blue, but the other colors confuse us. We would greatly appreciate the help of anyone who could give us information. Thank you for your time. Emily Dean E-mail: naedylime@hotmail.com ------------------------------------------------------------------ ************************************************************************ Dr. Arthur A.B. Bergen Department of Ophthalmogenetics The Netherlands Ophthalmic Research Institute (IOI) Royal Academy of Sciences of the Netherlands (KNAW) ** Snail-mail: ** ** FAX: ** ** E-mail: ** P.O.Box 12141 (+31)206916521 A.Bergen@IOI.KNAW.NL 1100 AC Amsterdam The Netherlands ************************************************************************