HUM-MOLGEN archive: BIOT: June 1997 various

New BIOTs! The BIOT section is open for requests and offers of information regarding biotechnology, molecular biology and molecular genetics You can reach over 4250 of your colleagues, and on average you may expect up to twenty replies to a single message. This service is absolutely FREE of charge. Help yourself by helping your colleagues! -Please send high quality messages only, including full name, address and purpose. -Please use the appropriate TOPIC subject heading in your message. -Please state non-trivial questions only. -Please reply by private E-mail only, unless your request is of general interest to the entire HUM-MOLGEN community Messages may be refused without further notification. Edward Wilcox Arthur Bergen (CALL editors) ************************************************************************ THIS MESSAGE CONTAINS THE FOLLOWING: 1) Request for E. coli strains 2) Request for an expert in RNA splicing 3) Request for help in multiplexing on a 377 ABI with Genescan software ************************************************************************ I would like to be working on the transfer of ampicillin resistance plasmids by Mating and conjugation methods. I need: Escherichia coli strain: a. Ampicillin sensitive (MIC Less than 4-8 Microgram/ml) b. F - (negative) c. Optionally: Lac - (negative) Nalidixic or/and Streptomycin Resistant Maybe strain CSH or something similar I have contacted: J H Miller (UCLA, USA) but with no response (yet) WHERE I CAN GET/BUY IT ??? Thanks and I hope to hear from you soon. Best regards, Dr. Kuntaman Microbiology Department, Medical Faculty Airlangga University Jl. Prof. Dr. Moestopo 47 Surabaya, Indonesia Fax: 062 031 522472 E-mail: "dr. Indrayana" <indranet@INDO.NET.ID> ************************************************************************ Dear colleagues Are you expert in RNA splicing?! In the course of mutation analysis on one of the mismatch repair genes, several unusual short transcripts were observed. Basically, RNA has been extracted from peripheral blood followed by RT-PCR. In some patients, shorter products were observed. On sequencing the short transcripts, it was found that the middle of one exon joins to the middle of another exon and the exons in between are missing. No alteration was found at splice junction sites or within the corresponding exons. One of these aberrant transcripts was found in four unrelated individuals. Another two mRNA deletions exhibit AAAGA before and after the breakage points. What is the possible explanation for these mRNA deletions? Any comments would be greatly appreciated. Thanks A. Davoodi E-mail: 9315831d@udcf.gla.ac.uk ************************************************************************ Dear Colleagues, Currently we are using the ABI377 and Genescan software for genotyping. We would like to develop a robust multiplex PCR for large scale projects. Does anybody have experience with multiplexing using the PTC100 by MJ Research ? All replies will be posted. Thank you. Min Ae Lee, M.D. Cardiovascular Division Brigham and Women's Hospital phone: 617-732 8174 fax: 617-264 6830 E-mail: mal@calvin.bwh.harvard.edu ************************************************************************ ************************************************************************ Edward Wilcox Laboratory of Molecular Genetics National Institute on Deafness and Other Communication Disorders 5 Research Court, 2A-19 Rockville, MD 20850-3227 USA Tel (301) 402-4162 FAX (301) 480-8019 E-mail edwilcox@pop.nidcd.nih.gov ************************************************************************ Dr. Arthur A.B. Bergen Department of Ophthalmogenetics The Netherlands Ophthalmic Research Institute (IOI) Royal Academy of Sciences of the Netherlands (KNAW) ** Snail-mail: ** ** FAX: ** ** E-mail: ** P.O.Box 12141 (+31)206916521 A.Bergen@IOI.KNAW.NL 1100 AC Amsterdam The Netherlands ************************************************************************