HUM-MOLGEN archive: BIOT: various August 1996

New BIOTechnology and Molecular biology issues; Subjects include protocols and material (Yac, cell lines, vectors) request; the latter on the basis of collaboration. There's also news from Biblio Biotech. Messages from a.o. Austria, USA, Netherlands, and Korea Please send **high quality messages only**; stating purpose and full reply adress. Given the fact that the network reaches now nearly 4000 subscribers from 63 countries, the flow of information increases; low-quality messages will thus be refused without notification. BIOT messages appear in a summarized fashion approximately once a month. Arthur Bergen ************************************************************************ >>Subject: diag >>Sender: Human Molecular Genetics Editors <ED-MOLGEN@HEARN.nic.SURFnet.nl> >>To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@HEARN.nic.SURFnet.nl> >>Reply-to: ANDRES CORNO <acorno@alc.es> >> >>acorno@alc.es (ANDRES CORNO) sent the following comments: >> >>------------------------------------------------------------ >>ANDRES CORNO >>DEPARTAMENTO DE PATOLOGIA Y CIRUGIA >>FACULTAD DE MEDICINA-CAMPUS SAN JUAN >>APDO 374 >>03080-ALICANTE -ESPAQA >> >> >> Alicante 12-8-1996 >> >> >>In my University ,recently received an Api 377, I would to know the best primers for amplified codon 12 -13 of the first exon of Human K-ras ,using paraffin-embedded sections of colorectal carcinomas and adenomas. >> >>I also would to know a software for drawing family trees. >> >>Thanks for your reply. >> >>TTT >>------------------------------------------------------------ >>Server protocol: HTTP/1.0 >>Remote host: inf186.alc.es >>Remote IP address: 194.179.105.186 >> > ************************************************************************ Subject: BIOT Date: Mon, 9 Sep 1996 22:59:08 +0200 Reply-to: Mike Goral <mgoral@bmclib2.bhs.org> From: Mike Goral <mgoral@bmclib2.bhs.org> Subject: call To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@NIC.SURFNET.NL> mgoral@bmclib2.bhs.org (Mike Goral) sent the following comments: ------------------------------------------------------------ Can anyone help me out with a workable protocol for DNA extraction from paraffin embedded tissue? ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: library.bhs.org Remote IP address: 199.92.147.2 ************************************************************************ Date: Sun, 1 Sep 1996 03:51:43 -0700 Reply-to: Human Molecular Genetics Editors <ED-MOLGEN@nic.SURFnet.nl> From: "Dyann K. Schmidel" <schmidel@IX.NETCOM.COM> Subject: The Biotech BiblioNet The Biotech BiblioNet is a bibliography of recently published biotech articles. New citations are added on the first day of each month and remain online for four months (older citations can be retrieved with NCBI's Entrez Medline). Many citations have direct links to online abstracts and to the email addresses of corresponding authors. You may browse through the topics or search for keywords. The Biotech BiblioNet also features collections of well organized, regularly updated links to the best molecular biology sites on the web. The Biotech BiblioNet is sponsored by: Jolley Consulting & Research, Inc. Lark Technologies, Inc. MicroSage Software VayTek, Inc. Best Regards, Dyann K. Schmidel, Ph.D. dyann@schmidel.com ************************************************************************** >From a.bergen@ioi.knaw.nlFri Sep 13 18:15:38 1996 Date: Fri, 13 Sep 1996 18:10:32 +0100 (MET) From: Arthur Bergen <a.bergen@ioi.knaw.nl> To: A.A.Bergen@AMC.UVA.NL Subject: BIOT Date: Sat, 31 Aug 1996 21:00:40 +0200 Reply-to: "m.bergmann, M.D." <m.bergmann@ic.ac.uk> From: "m.bergmann, M.D." <m.bergmann@ic.ac.uk> m.bergmann@ic.ac.uk (m.bergmann, M.D. ) sent the following comments: ------------------------------------------------------------ I am searching for an equivalent to the MACINTOSH program DNAstrider to create restriction maps, plasmid maps etc on a Windows 3.1 basis. In addition, a program for analyzing transcription factor binding sites would be very heplful. Any suggestions? thanks in advance M. Bergmann ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: Remote IP address: 155.198.240.73 ************************************************************************ Subject: BIOT: Gene therapy mike@canji.com (mike shepard) sent the following comments: ------------------------------------------------------------ canji is a subsidiary of schering-plough corp. we are serching for useful vectors for gene therpy, as well as new genes that could be used in the treatment of serious human diseases. we currently hae initiated worldwide clinical trials of p53 for gene therapy. ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: line13.cs9.electriciti.com Remote IP address: 198.5.214.23 ************************************************************************ Priority: normal Date: Tue, 27 Aug 1996 17:42:02 MET-1 Reply-to: R.deGroot@hli.azu.nl From: "Rolf P. de Groot" <R.deGroot@hli.azu.nl> Subject: Question Dear everybody We are trying to clone the promoter of a gene located on human chromosome 22 for some time now. However, we are still unsuccessfull. I wonder whether anybody could help us with a chromosome 22 specific genomic library (phage or cosmid). We would be happy to cooperate with anybody who could supply us with such a library. Thanks, Rolf P. de Groot Rolf P. de Groot Tel: (31)30 2507134 / 6754 Dept. Pulmonary Diseases Fax: (31)30 2542155 Room G03.550 Email: R.deGroot@hli.azu.nl University Hospital Utrecht Email2: pvlangen@worldaccess.nl PO Box 85500 3508 GA Utrecht The Netherlands ****************************************************************************** Date: Thu, 22 Aug 1996 11:55:39 -0700 Reply-to: Human Molecular Genetics Editors <ED-MOLGEN@NIC.SURFNET.NL> From: "dr. Indrayana" <indranet@INDO.NET.ID> Subject: help for "E. coli sex pili detection method" X-To: HUM-MOLGEN@HEARN.NIC.SURFNET.NL To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@NIC.SURFNET.NL> Dear Sir, Want to know about " simple detection methods of sex pili in Escherichia coli" Any information would be appreciated. Best regard, looking forward to hear from you. Yours sincerely, dr. Indrayana NS Email: indranet@server.indo.net.id Fax: 62 31 5322472 Airlangga School of Medicine Mayjen.Prof.Dr.Moestopo 47 Str. Surabaya Indonesia **************************************************************************** Date: Thu, 22 Aug 1996 20:59:09 +0000 Reply-to: Human Molecular Genetics Editors <ED-MOLGEN@NIC.SURFNET.NL> From: Carlo Gambacorti <GAMBACORTI@ICIL64.CILEA.IT> Subject: C --> A: I think more suitable for CALL; can you manage ? To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@NIC.SURFNET.NL> >Date: Mon, 12 Aug 1996 18:36:17 +0200 >From: ANDRES CORNO <acorno@ALC.ES> >Subject: diag >Sender: Human Molecular Genetics Editors <ED-MOLGEN@HEARN.nic.SURFnet.nl> >To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@HEARN.nic.SURFnet.nl> >Reply-to: ANDRES CORNO <acorno@alc.es> > >acorno@alc.es (ANDRES CORNO) sent the following comments: > >------------------------------------------------------------ >ANDRES CORNO >DEPARTAMENTO DE PATOLOGIA Y CIRUGIA >FACULTAD DE MEDICINA-CAMPUS SAN JUAN >APDO 374 >03080-ALICANTE -ESPAQA > > Alicante 12-8-199> >In my University ,recently received an Api 377, I would to know the best primers for amplified codon 12 -13 of the first exon of Human K-ras ,using paraffin-embedded sections of colorectal carcinomas and adenomas. > >I also would to know a software for drawing family trees. > >Thanks for your reply. > >TTT >------------------------------------------------------------ >Server protocol: HTTP/1.0 >Remote host: inf186.alc.es >Remote IP address: 194.179.105.186 > *************************************************************************** Date: Wed, 21 Aug 1996 09:46:08 -0600 Reply-to: Human Molecular Genetics Editors <ED-MOLGEN@NIC.SURFNET.NL> From: Andrew Wong <cwong@GPU.SRV.UALBERTA.CA> Subject: CALL: Obtain correct IMAGE cDNA clones This message was originally submitted by cwong@GPU.SRV.UALBERTA.CA to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear Human Molecular Geneticists: I have trouble to obtain some correct IMAGE cDNA clones. I ordered the clones through two different distributors: Genome System and ATCC. In the first time I ordered two clones from Genome System, one of them was a wrong clone. In another time I ordered seven clones from them, three of them were wrong. As a result, the percentage of getting a wrong clone from Genome System is close to 50. ATCC offers re-sequenced clone so they guarantee the clone is correct, but it seems that it takes forever to get a clone from them. I ordered one clone on June 12, 1996 but it still hasn't come yet. I'd like to know if anyone has similar problems to obtain IMAGE clones from these distributors and if you do, is there any way to get over the problems? Sincerely, Andrew Wong Graduate Student, University of Alberta ***************************************************************************** Date: Tue, 13 Aug 1996 16:13:17 +0200 Reply-to: Mike Goral <mgoral@bmclib2.bhs.org> From: Mike Goral <mgoral@bmclib2.bhs.org> Subject: call mgoral@bmclib2.bhs.org (Mike Goral) sent the following comments: ------------------------------------------------------------ I am looking for a source for follicular lymphoma cell line SU-DHL-6, or another t(14;18)(q32;q21) follicular lymphoma cell line. Can anyone help me? ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: library.bhs.org Remote IP address: 199.92.147.2 ************************************************************************** Date: Sun, 11 Aug 1996 17:14:39 +0900 Reply-to: Human Molecular Genetics Editors <ED-MOLGEN@nic.SURFnet.nl> From: dyshin@GERI4680.GERI.RE.KR Hi I am working at Korea Research Institute of Bioscience & Biotechnology as a junior staff. I am looking for a help about microdissection of human chromosome. If you have any information about who are doing microdissection of chromosome or which company or institute do microdissection service. thank you ***************************************************************************** From: c50326 <Kurt.Kofler@UIBK.AC.AT> Organization: University of Innsbruck, Austria Subject: YACs Could you help me please to find YACs for the chromosomal region 9q33-34? Thank you.