HUM-MOLGEN archive: BIOT: various April 1996

New BIOTechnology/molecular biology messages! your BIOT editors Martin Kennedy Arthur Bergen ***************************************************************************** >From mprimig@PASTEUR.FRWed 15:33:46 1996 I am currently trying to introduce a YAC (230kb, containing a lacZ-neomycine r construct) into C2 muscle cells using the polyethyleneglycol-mediated fusion technique of mammalian cells and yeast speroplasts. I keep getting G418 neo resistant colonies (at 750 micrograms of G418 per ml) that do not contain the YAC. Does anybody know anything about PEG mediated G418 tolerance and are there any good (detailed) protocols that yield true clones? I'd be very grateful for any suggestions! mike :-) ************************************************************************* >From huber@NT.IMP.UNIVIE.AC.ATWed 15:33:55 1996 From: "Lukas A. Huber" <huber@NT.IMP.UNIVIE.AC.AT> Mouse cDNA for ZO-1? We are presently working on protein transport and sorting in the oncogenic transformed epithelium. We could generate an mammary epithelial cell line transforemd with an induceable oncogene. For this we used a c-JunER fusion protein which is tightly controlled by estrogen. Activation of JunER by hormone resulted in a loss of epithelial polarity, a disruptio of intercellular junctions and normal barrier function and the formation of irregular multilayers. Loss of polarity involves also redistribution of both apical and basolateral proteins to th eentire plasmamembrane. These changes are completely reversible upon hormone withdrawel. I this mouse cells system we would like to study now the molecular organization of tight junctions before and after oncogenic transformation. We would therefore like to ask you whether anybody could help us with a mouse ZO-1 cDNA. We would be most appreciative for your help and reagents and I would certainly consider it as part of a real collaboration in case of any resulting publication. Lukas A. Huber I.M.P. Research Institute of Molecular Pathology Dr. Bohrgasse 7 A-1030 Wien, Austria Tel: 0043-1-79730-622 Fax: 0043-1-7987153 e-mail:HUBER@NT.IMP.UNIVIE.AC.AT ************************************************************** Univ. Doz. Dr. Lukas A. Huber I.M.P. Research Institute of Molecular Pathology Dr. Bohrgasse 7 A-1030 Wien, Austria Tel: 0043-1-79730-622 Fax: 0043-1-7987153 e-mail:HUBER@NT.IMP.UNIVIE.AC.AT ************************************************************** >From lxwei@ecmu.ihep.ac.cnWed May 15 15:34:04 1996 Date: Tue, 12 Mar 1996 11:03:45 +0000 From: Wei Lixin <lxwei@ecmu.ihep.ac.cn> To: A.A.Bergen@AMC.UVA.NL Subject: BIOT Dear Everyone: I am very interested in obtaining plasmids containing coding full sequences for Fas ligand, please let me know if there is someone out of there who can help. thankyou. Lixin Wei M.D. & Ph.D. Tumor Immunology & Gene Therapy Center Eastern Hepatobiliary Surgery Institute Changhai Hospital 174 Changhai Road Shanghai 200433 P.R.China yyy ********************************************************************** >From selim@CBU.BAYAR.EDU.TRWed May 15 15:34:18 1996 Date: Fri, 15 Mar 1996 14:26:32 +0300 From: Selim Uzunoglu <selim@CBU.BAYAR.EDU.TR> Reply to: Human Molecular Genetics Editors <ED-MOLGEN@nic.SURFnet.nl> To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@nic.SURFnet.nl> Subject: Diag Resent-Date: Fri, 15 Mar 1996 14:26:32 +0300 Resent-From: owner-ed-molgen@HEARN.NIC.SURFNET.NL Resent-To: A.A.Bergen@AMC.UVA.NL This message was originally submitted by selim@CBU.BAYAR.EDU.TR to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear, all, I am doing research on the molecular basis of familial monogenic hypercholesterolemia(FH)(LDL-receptor defect). I am looking for valid updated protocol for finding the homo or heterozygous FH patients among the patients with the atherosclerosis or cardiovascular disease problems. Is there any questionary list or the follow up strategy of biochemical tests and their evaluations to confirm that the biochemical findings of lipid measurements indicates that the patient is homo or heterozygous FH before embarking on DNA analysis? If anyone knows any information source on the subject of selective criteria, could you please send me information my below adress. Thank you very much to everybody in advance. Yours Sincerely Selim Uzunoglu *************************************************** *Dr. Selim UZUNOGLU (Ms.C;Ph.D in Medical Biology)* *Celal Bayar University Faculty of Medicine * *Medical Biology and Genetics Dept., Manisa-TURKEY* *W.Phone:+90 (236) 237 2928 * * (236) 237 2886 Ext:141 * *W.Fax :+90 (236) 237 2442 * *H.Phone:+90 (232) 388 9377 * * (232) 388 0208 * *H. Fax: +90 (232) 374 7754 * *mailto:selim@cbu.bayar.edu.tr * *URL http://www.bayar.edu.tr/~selim * *************************************************** ************************************************************************** >From bnkv@MUSICB.MCGILL.CAWed May 15 15:34:29 1996 Date: Sun, 1996 06:56:04 +0100 From: Jose Mejia <bnkv@MUSICB.MCGILL.CA> Subject: call Resent-Date: Sun, 17 Mar 1996 06:56:04 +0100 bnkv@musicb.mcgill.ca (Jose Mejia) sent the following comments: ------------------------------------------------------------ I want to know what is the best way to detect different alleles that con- tain dinucleotide repeats. They are 100-200 bp in size, and some of them are known to have 13 different alleles. Those alleles differ only in one couple of base pairs. Could you explain what might be a good size marker to detect such a small difference preciselly (ladder??) ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: mhew199.Res.McGill.CA Remote IP address: 132.216.32.199 **************************************************************************** >From pkusnier@IMMUNO.PAN.WROC.PLWed May 15 15:34:38 1996 Date: Mon, 15 Apr 1996 17:41:22 +0200 From: Piotr Kusnierczyk <pkusnier@IMMUNO.PAN.WROC.PL> Subject: sending mail to the list Resent-Date: Mon, 15 Apr 1996 17:41:22 +0200 This message was originally submitted by pkusnier@IMMUNO.PAN.WROC.PL to the HUM-MOLGEN list at NIC.SURFNET.NL. Dear colleagues, Does anybody know how to simply differentiate in PCR between human complement component 4 gene allele C4A*6 and all other alleles? I.e., what primer sequences and PCR conditions should be used to amplify selectively C4A*6 fragment from genomic DNA? I cannot fing such message in the MEDLINE and be very grateful for the information. Thank you in advance Piotr Piotr Kusnierczyk, Ph.D. Laboratory of Immunogenetics Ludwik Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences ul. Czerska 12, PL-53-114 Wroclaw, Poland tel. (+4871) 732274, ext. 226 fax (+4871) 679111 E-mail adress: pkusnier@immuno.pan.wroc.pl ************************************************************************* >From chownh@MAIL.NCKU.EDU.TWWed May 15 15:34:50 1996 Date: Fri, 19 Apr 1996 10:56:22 +0200 From: Nan-Haw Chow <chownh@MAIL.NCKU.EDU.TW> Subject: CALL, BIOT chownh@mail.ncku.edu.tw (Nan-Haw Chow) sent the following comments: ------------------------------------------------------------ I am looking for somebody who can give me the information regarding the microsatellite analysis of human tumor. We would like to know the technique detail and quality control of the procedure, if possible. ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: Remote IP address: 140.116.93.151 ************************************************************************* >From balld@MAIL.VT.EDUWed May 15 15:35:09 1996 Date: Tue, 23 Apr 1996 15:53:53 -0500 From: Don Ball <balld@MAIL.VT.EDU> Subject: Biotechnology 2001 (corrected Web Address) Resent-Date: Tue, 23 Apr 1996 15:53:53 -0500 BIOTECHNOLOGY 2001: A SYMPOSIUM FOR HIGH SCHOOL AND COLLEGE SCIENCE EDUCATORS Biotechnology 2001 is Virginia's first major biotechnology symposium for science educators. The goal of the symposium is to assist science educators in keeping up with scientific discoveries and related developments in biotechnology. New discoveries and their application to medicine, agriculture, and the environment are occurring at an astounding pace. Biotechnology will have far reaching implications for almost every aspect of our lives. However, keeping up to date is a daunting task for our educators. Therefore Virginia Tech's Fralin Biotechnology Center and the Division of Continuing Education have organized Biotechnology 2001. We encourage high school and college faculty to take advantage of this unique opportunity, but educators in all disciplines and other interested persons are also welcome to attend. The first day's program features Dr. Maxine Singer of the Carnegie Institution of Washington as keynote speaker along with other nationally and internationally recognized scientists. The symposium will be held at the newly renovated Hotel Roanoke and Conference Center on Friday, June 21. Participants are invited to visit Virginia Tech's Fralin Biotechnology Center on Saturday, June 22, for lectures by Virginia Tech scientists and tours of biotechnology laboratories. Each participant receives a CEU certificate crediting them for 1.2 Continuing Education Units. A CEU is a nationally recognized unit of measure which recognizes continuing education. They may be used for professional advancement or as evidence of increased abilities, but not for credit toward terminal degree programs. The Division of Continuing Education maintains a permanent, cumulative record of participation in all CEU offerings. Please check the link to BIOTECHNOLOGY 2001 program and registration form on the Fralin Biotechnology Center web site at: http://www.biotech.vt.edu LOCATION AND LODGING The conference will be held at The Hotel Roanoke and Conference Center located in downtown Roanoke at 110 Shenandoah Avenue. A covered walkway connects the hotel to the downtown business district where several unique shops and restaurants are located. A block of lodging rooms has been reserved at The Hotel Roanoke for Friday, June 21, at a special rate of $79 Single Occupancy (plus tax) and $89 Double Occupancy (plus tax). Please call The Hotel Roanoke and Conference Center at (540) 985-5900 and refer to this conference. Reservations must be made by June 5 to receive the discount rate. CONFERENCE SCHEDULE Friday, June 21, 1996 (held at The Hotel Roanoke & Conference Center) 8:00-9:00 Coffee and Registration Check-In 9:00-9:15 Welcome, Dr. Tracy Wilkins-Director of Virginia Tech Fralin Biotechnology Center and President of TechLab, Inc., Blacksburg, VA 9:15-10:15 Dr. Paula Gregory - Director of Education and Outreach for the National Center for Human Genome Research at National Institutes of Health, Bethesda, MD, Human Gene Therapy and Gene Delivery Systems 10:15-10:30 Break 10:30-11:30 Dr. Tracy Wilkins - Director of Virginia Tech Fralin Biotechnology Center and President of TechLab, Inc., Blacksburg, VA, Biotechnology, It's Not Just Molecular Biology Anymore 11:30-1:00 Buffet Luncheon 1:00-2:00 KEYNOTE ADDRESS: Dr. Maxine Singer - President, The Carnegie Institution of Washington, Washington, DC; Scientist Emeritus at National Institutes of Health, Bethesda, MD, Line-1 Sequences and Transposable Elements (or Jumping Genes) in Humans 2:00-3:00 Dr. Steven Shak - Project Team Leader of DNA Research and Director of Pulmonary Research at Genentech, Inc., San Francisco, CA, The Power of Biotechnology-A New Treatment for Cystic Fibrosis 3:00-3:15 Break 3:15-4:15 Dr. Doris Zallen - Director of the Choices and Challenges Program, Center for the Study of Science in Society, Virginia Tech, Blacksburg, VA, The Ethical Issues of Genetic Testing 4:15-5:15 Ms. Melissa Smrz - FBI Headquarters, Washington, DC, The Use of DNA in Forensic Science 5:15-6:15 Reception-Light hors d'oeuvres 6:30-7:30 Dr. Alvin Young - U.S. Department of Agriculture, Washington, DC, Biotechnology and New Products in Agriculture Saturday, June 22 (held at The Virginia Tech Fralin Biotechnology Center) (Numbers may be limited by seating available) 8:00-8:45 Coffee and Registration Check-In 8:45-9:30 Dr. Carole Cramer, Professor, Virginia Tech Department of Plant Pathology, Physiology and Weed Science, and CropTech Development Corporation, Blacksburg, VA, The Production of Human Biopharmaceutical Products from Transgenic Plants 9:30-10:15 Dr. Brenda Shirley, Assistant Professor, Virginia Tech Department of Biology, Blacksburg, VA, Molecular Biology of Flower Color 10:15-10:30 Break 10:30-11:15 Biotechnology Lab Activities Access Excellence Program - How to access this program developed by Genentech, Inc. and download lab activities via your computer. Presenters: Barbara Kolb, Ellen Mayo, Leslie Ann Pierce, Rebecca Sacra, Frank Taylor 11:15-12:00 Biotechnology on the Internet - Jim Kling and Rik Obiso 12:00-1:00 Lunch 1:00-3:00 Concurrent Tours (numbers may be limited) 1. Fralin Biotechnology Center Building 2. Lab of Dr. Carole Cramer - Transgenic Tobacco 3. Lab of Dr. Joe Falkinham - Mycobacterium tuberculosis 4. Lab of Dr. Dennis Dean - Nitrogen Fixation 5. Lab of Dr. Elizabeth Grabau - Transgenic Soybean 6. Lab of Dr. Kristi DeCourcy - Fralin Biotech Ctr. Outreach HOW TO REGISTER The registration fee for the conference is $65. The fee includes refreshment breaks, lunch and a reception at The Hotel Roanoke and Conference Center on Friday, June 21, handouts, and a lunch at the Virginia Tech Fralin Biotechnology Center on Saturday, June 22. Please complete the attached registration form and return with payment by May 21, 1996. After this date, registrations should be confirmed by phone with the Conference Registrar at (540) 231-5241. REFUND AND CANCELLATION POLICY: Requests for refunds are honored if received four full working days prior to the conference. However, substitutions are accepted at any time. The university may cancel or postpone any course because of insufficient enrollment or other unforeseen circumstances. If the course is cancelled or postponed, the university will refund registration fees, but cannot be held responsible for any other costs, charges, or expenses, including cancellation/change charges assessed by airlines or travel agencies. EXHIBITOR SPACE Exhibitor Fee is $100 plus $25 per six foot table. For further information about the conference, please contact Jack Lilly at the Division of Continuing Education at (540) 231-4849, fax-(540) 231-9886 or e-mail-Jacklily@vt.edu. FOR MORE INFORMATION For further information about the conference, please contact Barbara Duncan at the Division of Continuing Education at (540) 231-4849, fax-(540) 231-9886 or e-mail-Jacklily@vt.edu. CONFERENCE COMMITTEE Tracy Wilkins, Ph.D., Fralin Biotechnology Center, Virginia Tech, Blacksburg Rebecca Ross, Ed.D., Cave Spring High School, Roanoke Toby Horn, Ph.D., Thomas Jefferson High School for Science and Technology, Alexandria Barbara Kolb, James River High School, Buchanan John Kowalski, Ph.D., Roanoke Valley Governors School, Roanoke Carole Massart, Roanoke City Schools, Roanoke Ellen Mayo, Mills Godwin High School, Richmond Cheryl Lindeman, Ed.D., Central Virginia Governor's School, Lynchburg Lauri Goater-Cox, Ph.D., Carroll County High School, Hillsville Frank Taylor, Radford High School, Radford Leslie Pierce, Ph.D., Thomas Edison High School, Alexandria Kathy Frame, National Association of Biology Teachers, Reston Ellen Holtman, Virginia Western Community College, Roanoke Eric Collins, Wytheville Community College, Wytheville Norma Diehl, Piedmont Virginia Community College, Charlottesville Jill Losee-Hoehlein, Ed.D., Great Bridge High School, Chesapeake Valerie Cash, Fralin Biotechnology Center, Virginia Tech, Blacksburg Kristi DeCourcy, Ph.D., Fralin Biotechnology Center, Virginia Tech, Blacksburg Don Ball, Fralin Biotechnology Center, Virginia Tech, Blacksburg %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% Don Ball Fralin Center for Biotechnology VA Tech Blacksburg, VA 24061-0346 biotech@vt.edu 540 231-6934 540 231-7126 fax %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% ************************************************************************* >From jmejia@PO-BOX.MCGILL.CAWed May 15 15:35:26 1996 Date: Wed, 24 Apr 1996 07:31:55 +0200 From: Jose Mejia <jmejia@PO-BOX.MCGILL.CA> Subject: biot Resent-Date: Wed, 24 Apr 1996 07:31:55 +0200 jmejia@po-box.mcgill.ca (Jose Mejia) sent the following comments: ------------------------------------------------------------ Does it exist a restriction enzyme that cuts the sequence 5'ctag 3'? In this case, the sequence is flanked by T in the 5' and C in the 3' side. Thank you for your information. ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: mhew199.Res.McGill.CA Remote IP address: 132.216.32.199 ************************************************************************ >From R.Clarke@UNSW.EDU.AUWed May 15 15:36:01 1996 Date: Fri, 26 Apr 1996 08:50:57 +0200 From: Dr CLARKE <R.Clarke@UNSW.EDU.AU> Subject: CALL:CInverted PCR cloning Resent-Date: Fri, 26 Apr 1996 08:50:57 +0200 Resent-To: A.A.Bergen@AMC.UVA.NL R.Clarke@unsw.edu.au (Dr CLARKE) sent the following comments: ------------------------------------------------------------ We have sufficient sequence information to design primers for only one side of a chromosomal breakpoint. We want to sequence across the breakpoint without going through genomic cloning. The only way that appears feasible is to use inverted PCR after restriction enzyme digestion of genomic DNA and ligation to generate circular genomic fragments - then inverted PCR. Is this the only viable option - does any one have proven experience with this technique that might advise me on the things to watch. ------------------------------------------------------------ Server protocol: HTTP/1.0 Remote host: Remote IP address: 129.94.50.56