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  Bergen (ioi): BIOT: various April 1996  
   

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To: Multiple recipients of list HUM-MOLGEN <HUM-MOLGEN@NIC.SURFNET.NL>
Subject: BIOT: various April 1996
From: "Bergen (ioi)" <A.A.Bergen@AMC.UVA.NL>
Date: Wed, 15 May 1996 15:47:48 +0200

New BIOTechnology/molecular biology messages!

your BIOT editors

Martin Kennedy
Arthur Bergen


*****************************************************************************

>From mprimig@PASTEUR.FRWed  15:33:46 1996

I am currently trying to introduce a YAC (230kb, containing a
lacZ-neomycine r construct) into C2 muscle cells
using the polyethyleneglycol-mediated fusion technique of mammalian
cells and yeast speroplasts. I keep getting  G418 neo resistant
colonies (at 750 micrograms of G418 per ml) that do not contain the
YAC. Does anybody know anything about PEG mediated G418 tolerance and
are there any good (detailed) protocols that yield true clones?
I'd be very grateful for any suggestions!
mike :-)
*************************************************************************
>From huber@NT.IMP.UNIVIE.AC.ATWed  15:33:55 1996

From: "Lukas A. Huber" <huber@NT.IMP.UNIVIE.AC.AT>

 Mouse cDNA for ZO-1?

We are presently working on protein transport and sorting in the oncogenic
transformed epithelium.  We could generate an mammary epithelial cell line
transforemd with an induceable oncogene. For this we used a c-JunER fusion
protein which is tightly controlled by estrogen. Activation of JunER by
hormone resulted in a loss of epithelial polarity, a disruptio of
intercellular junctions and normal barrier function and the formation of
irregular multilayers. Loss of polarity involves also redistribution of
both apical and basolateral proteins to th eentire plasmamembrane. These
changes are completely reversible upon hormone withdrawel. I this mouse
cells system we would like to study now the molecular organization of tight
junctions before and after oncogenic transformation.
We would therefore like to ask you whether anybody could help us with a
mouse ZO-1 cDNA. We would be most appreciative for your help and reagents
and I would certainly consider it as part of a real collaboration in case
of any resulting publication.

Lukas A. Huber

I.M.P.
Research Institute of Molecular Pathology
Dr. Bohrgasse 7
A-1030 Wien, Austria
Tel: 0043-1-79730-622
Fax: 0043-1-7987153
e-mail:HUBER@NT.IMP.UNIVIE.AC.AT


**************************************************************

Univ. Doz. Dr. Lukas A. Huber

I.M.P.
Research Institute of Molecular Pathology
Dr. Bohrgasse 7
A-1030 Wien, Austria
Tel: 0043-1-79730-622
Fax: 0043-1-7987153
e-mail:HUBER@NT.IMP.UNIVIE.AC.AT

**************************************************************

>From lxwei@ecmu.ihep.ac.cnWed May 15 15:34:04 1996
Date: Tue, 12 Mar 1996 11:03:45 +0000
From: Wei Lixin <lxwei@ecmu.ihep.ac.cn>
To: A.A.Bergen@AMC.UVA.NL
Subject: BIOT


Dear Everyone:
I am very interested in obtaining plasmids containing coding full
sequences for Fas ligand, please let me know if there is someone out
of there who can help. thankyou.

Lixin Wei M.D. & Ph.D.
Tumor Immunology & Gene Therapy Center
Eastern Hepatobiliary Surgery Institute
Changhai Hospital
174 Changhai Road
Shanghai 200433
P.R.China
yyy
**********************************************************************
>From selim@CBU.BAYAR.EDU.TRWed May 15 15:34:18 1996
Date: Fri, 15 Mar 1996 14:26:32 +0300
From: Selim Uzunoglu <selim@CBU.BAYAR.EDU.TR>
Reply to: Human Molecular Genetics Editors <ED-MOLGEN@nic.SURFnet.nl>
To: Multiple recipients of list ED-MOLGEN <ED-MOLGEN@nic.SURFnet.nl>
Subject: Diag
Resent-Date: Fri, 15 Mar 1996 14:26:32 +0300
Resent-From: owner-ed-molgen@HEARN.NIC.SURFNET.NL
Resent-To: A.A.Bergen@AMC.UVA.NL

This  message  was  originally   submitted  by  selim@CBU.BAYAR.EDU.TR  to  the
HUM-MOLGEN list at  NIC.SURFNET.NL.

Dear, all,

I am doing research on the molecular basis of familial monogenic
hypercholesterolemia(FH)(LDL-receptor defect). I am looking for valid
updated protocol for finding the homo or heterozygous FH patients among
the patients with the atherosclerosis or cardiovascular disease
problems.
Is there any questionary list or the follow up strategy of biochemical
tests and their evaluations to confirm that the biochemical findings of
lipid measurements indicates that the patient is homo or
heterozygous FH before embarking on DNA analysis?
If anyone knows  any information source on the subject of selective
criteria, could you please send me information my below adress.
Thank you very much to everybody in advance.
Yours Sincerely
Selim Uzunoglu


***************************************************
*Dr. Selim UZUNOGLU (Ms.C;Ph.D in Medical Biology)*
*Celal Bayar University Faculty of Medicine       *
*Medical Biology and Genetics Dept., Manisa-TURKEY*
*W.Phone:+90 (236) 237 2928                       *
*            (236) 237 2886 Ext:141               *
*W.Fax  :+90 (236) 237 2442                       *
*H.Phone:+90 (232) 388 9377                       *
*            (232) 388 0208                       *
*H. Fax: +90 (232) 374 7754                       *
*mailto:selim@cbu.bayar.edu.tr                    *
*URL http://www.bayar.edu.tr/~selim               *
***************************************************
**************************************************************************

>From bnkv@MUSICB.MCGILL.CAWed May 15 15:34:29 1996
Date: Sun, 1996 06:56:04 +0100
From: Jose Mejia <bnkv@MUSICB.MCGILL.CA>
Subject: call
Resent-Date: Sun, 17 Mar 1996 06:56:04 +0100

bnkv@musicb.mcgill.ca (Jose Mejia) sent the following comments:

------------------------------------------------------------
I want to know what is the best way to detect different alleles that con-
tain dinucleotide repeats. They are 100-200 bp in size, and some of them
are known to have 13 different alleles. Those alleles differ only in one
couple of base pairs. Could you explain what might be a good size marker
to detect such a small difference preciselly (ladder??)
------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host: mhew199.Res.McGill.CA
Remote IP address: 132.216.32.199

****************************************************************************
>From pkusnier@IMMUNO.PAN.WROC.PLWed May 15 15:34:38 1996
Date: Mon, 15 Apr 1996 17:41:22 +0200
From: Piotr Kusnierczyk <pkusnier@IMMUNO.PAN.WROC.PL>
Subject: sending mail to the list
Resent-Date: Mon, 15 Apr 1996 17:41:22 +0200

This  message was  originally submitted  by pkusnier@IMMUNO.PAN.WROC.PL  to the
HUM-MOLGEN list at  NIC.SURFNET.NL.

Dear colleagues,
Does anybody know how to simply differentiate in PCR between human
complement component 4 gene allele C4A*6 and all other alleles? I.e., what
primer sequences and PCR conditions should be used to amplify selectively
C4A*6 fragment from genomic DNA? I cannot fing such message in the MEDLINE
and be very grateful for the information.

Thank you in advance
Piotr

Piotr Kusnierczyk, Ph.D.
Laboratory of Immunogenetics
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
Polish Academy of Sciences
ul. Czerska 12, PL-53-114 Wroclaw, Poland
tel. (+4871) 732274, ext. 226
fax (+4871) 679111
E-mail adress: pkusnier@immuno.pan.wroc.pl
*************************************************************************

>From chownh@MAIL.NCKU.EDU.TWWed May 15 15:34:50 1996
Date: Fri, 19 Apr 1996 10:56:22 +0200
From: Nan-Haw Chow <chownh@MAIL.NCKU.EDU.TW>
Subject: CALL, BIOT

chownh@mail.ncku.edu.tw (Nan-Haw Chow) sent the following comments:

------------------------------------------------------------
I am looking for somebody who can give me the information regarding
the microsatellite analysis of human tumor. We would like to know
the technique detail and quality control of the procedure, if possible.
------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host:
Remote IP address: 140.116.93.151

*************************************************************************
>From balld@MAIL.VT.EDUWed May 15 15:35:09 1996
Date: Tue, 23 Apr 1996 15:53:53 -0500
From: Don Ball <balld@MAIL.VT.EDU>
Subject: Biotechnology 2001 (corrected Web Address)
Resent-Date: Tue, 23 Apr 1996 15:53:53 -0500

BIOTECHNOLOGY 2001:  A SYMPOSIUM FOR HIGH SCHOOL AND COLLEGE SCIENCE EDUCATORS

Biotechnology 2001 is Virginia's first major biotechnology symposium for
science educators.  The goal of  the symposium is to assist science
educators in keeping up with scientific discoveries and related
developments in biotechnology.  New discoveries and their application to
medicine, agriculture, and the environment are occurring at an astounding
pace.  Biotechnology will have far reaching implications for almost every
aspect of our lives.  However, keeping up to date is a daunting task for
our educators.  Therefore Virginia Tech's Fralin Biotechnology Center and
the Division of Continuing Education have organized Biotechnology 2001.  We
encourage high school and college faculty to take advantage of this unique
opportunity, but educators in all disciplines and other interested persons
are also welcome to attend.

The first day's program features Dr. Maxine Singer of the Carnegie
Institution of Washington as keynote speaker along with other nationally
and internationally recognized scientists.  The symposium will be held at
the newly renovated Hotel Roanoke and Conference Center on Friday, June 21.
Participants are invited to visit Virginia Tech's Fralin Biotechnology
Center on Saturday, June 22, for lectures by Virginia Tech scientists and
tours of  biotechnology laboratories.

Each participant receives a CEU certificate crediting them for 1.2
Continuing Education Units.  A CEU is a nationally recognized unit of
measure which recognizes continuing education.  They may be used for
professional advancement or as evidence of increased abilities, but not for
credit toward terminal degree programs.  The Division of Continuing
Education maintains a permanent, cumulative record of participation in all
CEU offerings.

Please check the link to BIOTECHNOLOGY 2001 program and registration form
on the Fralin Biotechnology Center web site at:  http://www.biotech.vt.edu

LOCATION AND LODGING

The conference will be held at The Hotel Roanoke and Conference Center
located in downtown Roanoke at 110 Shenandoah Avenue. A covered walkway
connects the hotel to the downtown business district where several unique
shops and restaurants are located.

A block of lodging rooms has been reserved at The Hotel Roanoke for Friday,
June 21, at a special rate of $79 Single Occupancy (plus tax) and $89
Double Occupancy (plus tax).  Please call The Hotel Roanoke and Conference
Center at (540) 985-5900 and refer to this conference. Reservations must be
made by June 5 to receive the discount rate.


CONFERENCE SCHEDULE

Friday, June 21, 1996     (held at The Hotel Roanoke & Conference Center)


8:00-9:00       Coffee and Registration Check-In

9:00-9:15       Welcome, Dr. Tracy Wilkins-Director of Virginia Tech Fralin
Biotechnology Center and President of TechLab, Inc., Blacksburg, VA

9:15-10:15      Dr. Paula Gregory - Director of Education and Outreach for
the National Center for Human Genome Research at National Institutes of
Health, Bethesda, MD, Human Gene Therapy and Gene Delivery Systems

10:15-10:30     Break

10:30-11:30     Dr. Tracy Wilkins - Director of Virginia Tech Fralin
Biotechnology Center and President of TechLab, Inc., Blacksburg, VA,
Biotechnology, It's Not Just Molecular Biology Anymore

11:30-1:00      Buffet Luncheon

1:00-2:00       KEYNOTE ADDRESS:
        Dr. Maxine Singer - President, The Carnegie Institution of
Washington, Washington, DC; Scientist Emeritus at National Institutes of
Health, Bethesda, MD, Line-1 Sequences and Transposable Elements (or
Jumping Genes) in Humans

2:00-3:00       Dr. Steven Shak - Project Team Leader of DNA Research and
Director of Pulmonary Research at Genentech, Inc., San Francisco, CA, The
Power of Biotechnology-A New Treatment for Cystic Fibrosis

3:00-3:15       Break

3:15-4:15       Dr. Doris Zallen - Director of the Choices and Challenges
Program, Center for the Study of Science in Society, Virginia Tech,
Blacksburg, VA, The Ethical Issues of Genetic Testing

4:15-5:15       Ms. Melissa Smrz - FBI Headquarters, Washington, DC, The
Use of DNA in Forensic Science

5:15-6:15       Reception-Light hors d'oeuvres

6:30-7:30       Dr. Alvin Young -  U.S. Department of Agriculture,
Washington, DC, Biotechnology and New Products in Agriculture



Saturday, June 22      (held at The Virginia Tech Fralin Biotechnology Center)

(Numbers may be limited by seating available)

8:00-8:45       Coffee and Registration Check-In

8:45-9:30       Dr. Carole Cramer,  Professor, Virginia Tech Department of
Plant Pathology, Physiology and Weed Science, and CropTech Development
Corporation, Blacksburg, VA, The Production of Human Biopharmaceutical
Products from Transgenic Plants

9:30-10:15      Dr. Brenda Shirley, Assistant Professor, Virginia Tech
Department of Biology, Blacksburg, VA, Molecular Biology of Flower Color

10:15-10:30     Break

10:30-11:15     Biotechnology Lab Activities
        Access Excellence Program - How to access this program developed by
Genentech, Inc. and download lab activities via your computer.  Presenters:
Barbara Kolb, Ellen Mayo, Leslie Ann Pierce, Rebecca Sacra, Frank Taylor

11:15-12:00     Biotechnology on the Internet - Jim Kling and Rik Obiso

12:00-1:00      Lunch

1:00-3:00       Concurrent Tours (numbers may be limited)
        1.      Fralin Biotechnology Center Building
        2.      Lab of Dr. Carole Cramer - Transgenic Tobacco
        3.      Lab of Dr. Joe Falkinham - Mycobacterium tuberculosis
        4.      Lab of Dr. Dennis Dean - Nitrogen Fixation
        5.      Lab of Dr. Elizabeth Grabau - Transgenic Soybean
        6.      Lab of Dr. Kristi DeCourcy - Fralin Biotech Ctr. Outreach


HOW TO REGISTER

The registration fee for the conference is $65.  The fee includes
refreshment breaks, lunch and a reception at The Hotel Roanoke and
Conference Center on Friday, June 21, handouts, and a lunch at the Virginia
Tech Fralin Biotechnology Center on Saturday, June 22.

Please complete the attached registration form and return with payment by
May 21, 1996. After this date, registrations should be confirmed by phone
with the Conference Registrar at (540) 231-5241.

REFUND AND CANCELLATION POLICY:
Requests for refunds are honored if received four full working days prior
to the conference. However, substitutions are accepted at any time. The
university may cancel or postpone any course because of insufficient
enrollment or other unforeseen circumstances. If the course is cancelled or
postponed, the university will refund registration fees, but cannot be held
responsible for any other costs, charges, or expenses, including
cancellation/change charges assessed by airlines or travel agencies.


EXHIBITOR SPACE

Exhibitor Fee is $100 plus $25 per six foot table.  For further information
about the conference, please contact Jack Lilly at the Division of
Continuing Education at (540) 231-4849, fax-(540) 231-9886 or
e-mail-Jacklily@vt.edu.


FOR MORE INFORMATION

For further information about the conference, please contact Barbara Duncan
at the Division of Continuing Education at (540) 231-4849, fax-(540)
231-9886 or e-mail-Jacklily@vt.edu.

CONFERENCE COMMITTEE

Tracy Wilkins, Ph.D., Fralin Biotechnology Center, Virginia Tech, Blacksburg

Rebecca Ross, Ed.D., Cave Spring High School, Roanoke

Toby Horn, Ph.D., Thomas Jefferson High School for Science and Technology,
Alexandria

Barbara Kolb, James River High School, Buchanan

John Kowalski, Ph.D., Roanoke Valley Governors School, Roanoke

Carole Massart, Roanoke City Schools, Roanoke

Ellen Mayo, Mills Godwin High School, Richmond

Cheryl Lindeman, Ed.D., Central Virginia Governor's School, Lynchburg

Lauri Goater-Cox, Ph.D., Carroll County High School, Hillsville

Frank Taylor,  Radford High School, Radford

Leslie Pierce, Ph.D., Thomas Edison High School, Alexandria

Kathy Frame, National Association of Biology Teachers, Reston

Ellen Holtman, Virginia Western Community College, Roanoke

Eric Collins, Wytheville Community College, Wytheville

Norma Diehl, Piedmont Virginia Community College, Charlottesville

Jill Losee-Hoehlein, Ed.D., Great Bridge High School, Chesapeake

Valerie Cash, Fralin Biotechnology Center, Virginia Tech, Blacksburg

Kristi DeCourcy, Ph.D., Fralin Biotechnology Center, Virginia Tech, Blacksburg

Don Ball, Fralin Biotechnology Center, Virginia Tech, Blacksburg

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

Don Ball
Fralin Center for Biotechnology
VA Tech
Blacksburg, VA 24061-0346

biotech@vt.edu

540 231-6934
540 231-7126  fax

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%


*************************************************************************

>From jmejia@PO-BOX.MCGILL.CAWed May 15 15:35:26 1996
Date: Wed, 24 Apr 1996 07:31:55 +0200
From: Jose Mejia <jmejia@PO-BOX.MCGILL.CA>
Subject: biot
Resent-Date: Wed, 24 Apr 1996 07:31:55 +0200

jmejia@po-box.mcgill.ca (Jose Mejia) sent the following comments:

------------------------------------------------------------
Does it exist a restriction enzyme that cuts the sequence 5'ctag 3'? In this case, the sequence is flanked by T in the 5' and  C in the 3' side. Thank you for your information.
------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host: mhew199.Res.McGill.CA
Remote IP address: 132.216.32.199

************************************************************************

>From R.Clarke@UNSW.EDU.AUWed May 15 15:36:01 1996
Date: Fri, 26 Apr 1996 08:50:57 +0200
From: Dr CLARKE <R.Clarke@UNSW.EDU.AU>
Subject: CALL:CInverted PCR cloning
Resent-Date: Fri, 26 Apr 1996 08:50:57 +0200
Resent-To: A.A.Bergen@AMC.UVA.NL

R.Clarke@unsw.edu.au (Dr CLARKE) sent the following comments:

------------------------------------------------------------
We have sufficient sequence information to design primers for only one side
of a chromosomal breakpoint. We want to sequence across the breakpoint
without going through genomic cloning. The only way that appears feasible
is to use inverted PCR after restriction enzyme digestion of genomic
DNA and ligation to generate circular genomic fragments - then inverted
PCR. Is this the only viable option - does any one have proven experience with
this technique that might advise me on the things to watch.
------------------------------------------------------------
Server protocol: HTTP/1.0
Remote host:
Remote IP address: 129.94.50.56


   
 
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