HUM-MOLGEN archive: BIOT: M'satellites, fluor sequencers etc

Date sent: 2-OCT-1995 10:42:49 This message contains 5 parts. My apologies for the delayed posting of the first 2 messages (MK). 1. DNA Stutter bands 2. Argon laser replacement 3. Li-Cor infra-red laser sequencer 4. ABI 373A fluor. primers for microsatellite analysis 5. Wanted: Internships for Biotech graduate Martin Kenendy/Arthur Bergen BIOT Topic editors, HUM-MOLGEN 8< ======================= cut here ======================= >8 Date: Thu, 21 Sep 1995 11:14:29 GMT re: DNA Stutter bands (Martin Perlin) I am currently developing a method for rapid screening of markers using DNA pooling. I have also used computer software to eliminate stutter bands and differential amplification. I would be interested in copies of your paper detailing the expressions you used to subtract stutter. Thanks, Jo Daniels email: WMGJKD@cardiff.ac.uk 8< ======================= cut here ======================= >8 Date: Thu, 21 Sep 1995 14:35:19 +0200 From: Paul Betts <paul_b@BIOTEK.MCB.UCONN.EDU> Subject: argon laser replacement Hello, I run a core nucleic acids facility and the argon laser on my automated sequencer has just discovered its own mortality. I have been told by the manufacturer that in addition to the laser I need to replace the power source. I was told, that without the power supply change, a new laser would probably expire within a month. The difference is more than acedemic as to replace the laser is $1,500 - $2,000 whereas including the source brings the cost to $11,000.00. This sounds like highway robbery to me. Is this for real? I would like to know if others have had this experience with their ABI or ALF sequencers. 8< ======================= cut here ======================= >8 Date: Fri, 22 Sep 1995 08:32:14 +0200 From: "Clemens R. Mueller" <crm@BIOZENTRUM.UNI-WUERZBURG.DE> Subject: LI-COR sequencer Hallo, does anyone have experience with the LI-COR infra-red laser sequencer? We are interested in sequencing and fragment detection (e.g. microsatellites, SSCP etc.). Thank you! ********************************************* * Clemens R. Mueller, Ph.D. * * Institut fuer Humangenetik * * der Universitaet Wuerzburg * * Biozentrum, Am Hubland * * D-97074 Wuerzburg, Germany * * * * Tel: (+)49-931-888-4063 * * Fax: (+)49-931-888-4069 * * e-mail: crm@biozentrum.uni-wuerzburg.de * ********************************************* 1895 - 1995 100 years X-rays discovered by W.C. Roentgen in 1895 at Wuerzburg University 8< ======================= cut here ======================= >8 Date: Sat, 30 Sep 1995 12:34:06 -0400 From: "Dr. Paula Strasberg" <pstras@RESUNIX.RI.SICKKIDS.ON.CA> Subject: reply to sep 14 query by Agnes Hi- To Agnes >This is in response to your query, dated Sept. 14, 1995. We use the ABI 373A with fluorescent primers for: 1. Duchenne/Becker Muscular Dystrophy multiplex quantitative PCR (2 multiplex reactions, 9 and 10 bands each) for carrier detection, and for deletion and duplication analysis in patients. 2. Fragile X analysis of query affected patients and carrier detection in females, or normal transmitting males- size of repeat is measured. 3. Microsatellite analysis for identity/zygosity testing, bone marrow transplant followups/12 markers. 4. SMA quantitative analysis of deletion of the SMN gene (deleted in 98.6% of affecteds), to detect carriers and affected individuals and use this information for diagnosis- this test is in the process of being evaluated. Dr. Paula Strasberg Associate Director, Molecular Diagnostic Laboratory The Hospital for Sick Children Asst. Professor, Dept. of Clinical Biochemistry University of Toronto Phone 813-6751, FAX 813-5086, email pstras@sickkids.on.ca 8< ======================= cut here ======================= >8 Date: Sun, 1 Oct 1995 11:53:15 EST From: "Miller, Angela" <F409@MUSIC.FERRIS.EDU> Subject: Internships I am interested in finding an internship. I am currently in the Biotechnology Program at Ferris State University in Michigan. I really would like to have an intern and would be available to begin one in mid-May. The classes I'm taking focus on: recombinant DNA, tissue culture, and advanced protein isolation techniques. I would be interested in an intern in any of those areas especially tissue culture. If you have any information that you think could help me my e-mail address is: F409@MUSIC.FERRIS.EDU Thanks, Angie Miller 8< ======================= THE END ======================= >8 Cheers, Martin NNNN NN Martin A Kennedy (E-mail = mkennedy@chmeds.ac.nz) ZZZZZZZ NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ NN NN NN Christchurch School of Medicine ZZZ NN NNNN Christchurch, New Zealand ZZZZZZZ Phone (64-3)364-0880 Fax (64-3)364-0750