HUM-MOLGEN archive: BIOT: PCR genotyping

Note from the editors: This BIOT message contains one submessage: 1) technical info concerning PCR genotyping wanted The BIOT TOPIC of HUM-MOLGEN is open for FREE communication concerning topics of interest of molecular biologist en geneticists. Communication includes: new techniques, products, etc. help and advice wanted for technical protocols or methods, finding and offering of funds, etc. Unlike other sections on HUM-MOLGEN, commercial mesasages, although strictly regulated, are allowed on the BIOT TOPIC. The BIOT editors Martin Kennedy Arthur Bergen ************************************************************************* This message was originally submitted by mcbtayhn@LEONIS.NUS.SG to the HUM-MOLGEN list at NIC.SURFNET.NL Hi I'm interested in doing PCR genotyping and would like some advice. I've previously done PCR genotyping of dinucleotide repeats using 32P endlabeled primers which worked well but I'd like to explore non-radioactive methods. Ag staining has been suggested and I'd be grateful for any useful tips on this. Also, I used to size the alleles using a sequencing ladder but I wonder if there's any other way. The PCR product I'm expecting would be abt 250 bp with 2bp differences between alleles. Thanks Agnes