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To: Multiple recipients of list HUM-MOLGEN <HUM-MOLGEN@NIC.SURFNET.NL>
Subject: BIOT: Dynabeads
From: Arthur Bergen <bergen@AMC.UVA.NL>
Date: Fri, 9 Jun 1995 12:29:21 +0100

Subj:   Use of Dynabeads with automated sequencing

This message was  originally submitted by dgill@UNM.EDU to  the HUM-MOLGEN list
at NIC.SURFNET.NL.

I have been using Dynabeads (paramagnetic beads coated w/ streptavidin)
to isolate ss PCR product and then sequence manualy. We just got an
automated sequencer (ABI 373a) and I am having problems getting the same
PCR products to sequence on the ABI. I am using the Sequenase dye
terminator and Taq cycle dye terminator kits. Has anyone tried these with
dynabeads? The ABI techs were not much help.... I would appreciate any
replies. Thanks.

David L. Gillespie           \   /\ /\   /\ /\   /\ /\    Office:
dgill@hydra.unm.edu          |\ /||/||\ /||/||\ /||/||\   (505) 277-8343
University of New Mexico    \||/||/ \||/||/ \||/||/ \|    FAX:
Dept. of Cell Biology        \/ \/   \/ \/   \/ \/   \    (505) 277-9494


   
 
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