1. Edition - May 2004 - 55.- Euro / 81.- SFR 2004. XVI, 256 Pages, Softcover ISBN 3-527-30444-4
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A broad experimental introduction considering all essential aspects of enzymology, containing a theoretical introduction together with practical protocols. Essential reading for all scientists and practitioners working with enzymes. From the contents
INTRODUCTION ENZYME NOMENCLATURE ENZYME REACTIONS Theory of the enzyme reaction Michaelis-Menten equation Theory of the enzyme test Theory of coupled enzyme reactions Substrate determination Instrumental aspects Enzyme assays Diverse methods Enzyme immunoassays BINDING MEASUREMENTS Irreversible, reversible, specific and unspecific binding Binding measurement by size discrimination Spectroscopic techniques Other methods for binding measurements ENZYMES IN TECHNICAL APPLICATIONS Principles of enzyme immobilisation Methods for enzyme immobilisation Analysis of immobilised enzymes Enzyme reactors Biosensors Immobilized enzymes in therapy APPENDIX List of enzymes according to EC numbers
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