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Schepers. 2004. RNA Interference in Practice. 1st ed.

  June, 28 2005 17:37
new and historical books in bioscience and medicine
1. Edition - October 2004
99.- Euro / 158.- SFR
2004. X, 326 Pages, Hardcover
ISBN 3-527-31020-7 - Wiley-VCH, Weinheim

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Short description

This hands-on guide brings the power of targeted gene silencing to any lab with the basic equipment for handling nucleic acids.
In step-by-step protocols you will learn how RNAi works in worms, flies and mammals and how to design the most efficient RNAi constructs.
All protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments.

Sample Chapter

From the contents

- Mechanism of RNAi
- RNAi as a Tool for Functional Genomics

RNAi in C.elegans
- Practical Application
- Target Evaluation
- Synthesis and Delivery of dsRNA
- Genome-wide Screening

RNAi in Drosophila
- Practical Application
- Synthesis and Delivery of dsRNA
- RNAi in Cell Lines

RNAi in mammals
- Transient RNAi in Cell Culture
- Design and Synthesis of siRNAs
- Delivery of siRNAs into Cells
- Analysis and Validation of siRNAs
- RNAi with Hairpin RNAs
- Inducible RNAi
- RNAi in mice
- Screening Methods

RNAi Resources
- Web Resources
- List of Suppliers
- Glossary of Terms

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