1. Edition - October 2004
99.- Euro / 158.- SFR
2004. X, 326 Pages, Hardcover
ISBN 3-527-31020-7 - Wiley-VCH, Weinheim
-> Wiley
-> Amazon Short description This hands-on guide brings the power of targeted gene silencing to any lab with the basic equipment for handling nucleic acids.
In step-by-step protocols you will learn how RNAi works in worms, flies and mammals and how to design the most efficient RNAi constructs.
All protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments.
Sample Chapter
From the contents
Introduction
- Mechanism of RNAi
- RNAi as a Tool for Functional Genomics RNAi in C.elegans
- Practical Application
- Target Evaluation
- Synthesis and Delivery of dsRNA
- Genome-wide Screening RNAi in Drosophila
- Practical Application
- Synthesis and Delivery of dsRNA
- RNAi in Cell Lines RNAi in mammals
- Transient RNAi in Cell Culture
- Design and Synthesis of siRNAs
- Delivery of siRNAs into Cells
- Analysis and Validation of siRNAs
- RNAi with Hairpin RNAs
- Inducible RNAi
- RNAi in mice
- Screening Methods RNAi Resources
- Web Resources
- List of Suppliers
- Glossary of Terms
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