Birren B et al. 1997. Genome Analysis: Lab. Manual: Analyzing DNA (selected chapter)

Genome Analysis : A Laboratory Manual : Analyzing DNA (Vol 1)
by Bruce Birren (Editor), Eric D. Green (Editor), Sue Klapholz (Editor)

Cold Spring Harbor Laboratory
ISBN: 0879694963
December 1997

Book Description

An Assuming only a basic knowledge of molecular biology, volume one describes basic techniques in genome analysis that are applicable to most of the experimental methods appearing in forthcoming manuals (Detecting Genes, Cloning Systems, and Mapping Genomes). The first two chapters provide basic protocols for isolating genomic DNA and performing standard manipulations, as well as protocols for isolating, manipulating, and analyzing high-molecular-weight DNA. Subsequent chapters cover PCR-based applications; large-scale DNA sequencing, including protocols for dideoxy-mediated sequencing and specific methods for shotgun and directed sequencing strategies; and a review of the most critical methods<-->those associated with analyzing and accessing genomic information, particularly sequence data. Plastic comb binding. Book News, Inc.®, Portland, OR

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Selected chapter:
Chapter 7: Computational Analysis of DNA and Protein Sequences