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virtual library in human genetics and molecular biology

EDITORIAL

The End of the Beginning: The Race to Begin Human Genome Sequencing

REVIEWS

Lessons Learned, Promises Kept: A Biologist's Eye View of the Genome Project
Shirley M. Tilghman 773

An Approach to High-throughput Genotyping
Jeff M. Hall, Carrie A. LeDuc, Andy R. Watson, and Alan H. Roter 781

RESEARCH

Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery
Maria de Fatima Bonaldo, Gregory Lennon, and Marcelo Bento Soares 791

Generation and Analysis of 280,000 Human Expressed Sequence
Tags LaDeana Hillier, Greg Lennon, Michael Becker, M. Fatima Bonaldo, Brandi Chiapelli, Stephanie Chissoe, Nicole Dietrich, Treasa DuBuque, Anthony Favello, Warren Gish, Maria Hawkins, Monica Hultman, Tamara Kucaba, Michelle Lacy, Maithao Le, Nha Le, Elaine Mardis, Bradley Moore, Matthew Morris, Jeremy Parsons,Christa Prange, Lisa Rifkin, Theresa Rohlfing, Kurt Schellenberg, M. Bento Soares, Fang Tan, Jean Thierry-Meg, Evanne Trevaskis, Karen Underwood, Patricia Wohldman, Robert Waterston, Richard Wilson, and Marco Marra 807

Toward the Development of a Gene Index to the Human Genome: An Assessment of the Nature of High-throughput EST Sequence Data
Jeffrey S. Aaronson, Barbara Eckman, Richard A. Blevins, Joseph A. Borkowski, Joseph Myerson, Shahid Imran, and Keith O. Elliston 829

Comparative Analysis of 1196 Orthologous Mouse and Human Full-length mRNA and Protein Sequences
Wojciech Makatowski, Jinghui Zhang, and Mark S. Boguski 846

Intron 17 of the Human Retinoblastoma Susceptibility Gene Encodes an Actively Transcribed G Protein-coupled Receptor
Gene Herbert Herzog, Karen Darby, Yvonne J. Hort, and John Shine 858

Fine Mapping of the Autosomal Dominant Juvenile Open Angle Glaucoma (GLC1A) Region and Evaluation of Candidate Genes
Sara L.F. Sunden, Wallace L.M. Alward, Brian E. Nichols, Tanya R. Rokhlina, Arne Nystuen, Edwin M. Stone, and Val C. Sheffield 862

Tracing an Ancestral Mutation: Genealogical and Haplotype Analysis of the Infantile Onset Spinocerebellar Ataxia Locus
Teppo Varilo, Kaisu Nikali, Anu Suomalainen, Tuula Lönnqvist, and Leena Peltonen 870

LETTERS

Mutations in Sheep Microsatellites
Allan M. Crawford and Rachel P. Cuthbertson 876

Cloning of the Murine Homolog of the Ocular Albinism Type 1 (OA1) Gene: Sequence, Genomic Structure, and Expression Analysis in Pigment Cells
Maria Teresa Bassi, Barbara Incerti, David J. Easty, Elena V. Sviderskaya, and Andrea Ballabio 880

GENOME METHODS

Use of Asymmetric PCR to Generate Long Primers and Single-stranded DNA for Incorporating Cross-linking Analogs into Specific Sites in a DNA Probe
Christine I. Wooddell and Richard R. Burgess 886

Sensitivity, Reproducibility, and Accuracy in Short Tandem Repeat Genotyping Using Capillary Array Electrophoresis
Elaine S. Mansfield, Marina Vainer, Shellee Enad, David L. Barker, Dennis Harris, Eric Rappaport, and Paolo Fortina 893

Product News 904

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