Rumen Pro-Plasmid Info Enterprises of Canada

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C.-B.
Canada
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Description:

NON-GMO, GENETICALLY REGULATORY ORGANISMAL (GRO) MICROBIAL MODULATION OF RUMEN FERMENTATION FOR DAIRY & BEEF PRODUCTION. 

A. MODULATING ENZYME ACTIVITY AND C-SUBSTRATES PRODUCTION IN ACETATE-TYPE FERMENTATION MICROBIAL PATHWAYS VIA THEIR PLASMID REGULONS.

For acetate-type fermentation tailored for dairy milk production, it is recommended to slow proteolytic microbes and their expression of proteases to optimize protein escape from the rumen and also to slow propionic acid fermentation; up fibrolytic microbes and their fibrolytic enzymes and up acetic acid type fermentation to acetate endproduct via upping the production of C-substrates that are rate-limiting for fermentation of acetate, viz. the acetyl-CoA pathway involving the spp.: Prevotella spp. in proportion to total VFA production, Fibrobacter succinogenes and Selenomonas ruminantium proportionate to fibre fermentation and VFA production (linked to H2(g) and acetate dynamics) and Lactobacillus spp. (buchneri; acidophilus) responsible for conversion of lactose to acetate.

B. MODULATING ENZYME ACTIVITIES AND C-SUBSTRATES PRODUCTION IN ACETATE-TYPE FERMENTATION MICROBIAL PATHWAYS VIA THEIR PLASMID REGULONS.

For propionate-type fermentation for meat production it is recommended to slightly slow proteolytic microbes and their proteases, slowing N-degradation and NH3 loss to optimize protein (P) escape to the small intestines; up fibrolytic microbes and their fibrolytic enzymes and up amylolytic microbes and their amylolytic enzymes to up fermentation to propionate through upping production of C-substrates that are rate-limiting for fermentation of propionic acid involving the: Acetyl-CoA pathway with Prevotella spp. proportionate to the total VFA production, Succinate pathway in Fibrobacter succinogenes and Selenomonas ruminantium and the Acrylate pathway responsible for converting lactate to propionate with Megasphaera elsdenii. 

C.  MODEL FEEDING SYSTEMS FOR DAIRY PRODUCTION WITH LOW-QUALITY AND HIGH-QUALITY FEEDS (LQFDS & HQFDS) FOR TEMPERATE AND TROPICAL COUNTRIES.

It is recommended that 2 schemas for feeding systems for dairy water buffaloes and Italian crossbred bufffaloes be introduced. a) LQFDS which are more sustainable in point be used such as whole plant corn silage, sugarcane tops (SCT) silage, rice straw, and giant Pangola grasses for acetate and milk. b) HQFDS from aquaculture and phytopharm intensive systems for so-called "XL-GRO" seagrasses and seed protein extract concentrates (SPEC) protein concentrates for Hi-bred holsteins (GRO class cattle) and Hi-bred, Crossbred Italian x Native crosses (GRO class cattle) for dairy production in our Land Food Systems (LFS) in the future.  This is intensive in the sense that aquaculure operators in the prototype plants are highly mechanized and semi-automated by handlers dependent on industrialization or semi-industrialization in variou country type settings. Ensilage in many cases are adopted which results in better managed logistics with feeding roughage or fodders with twice feeding during the year, with once or twice harvest times including feeding over off season (Winter time) and growing in season (warmer seasons).  E. g. of countries where this can be used are in the USA, Canada, Queensland (AU), Brazil, the Philippines and Pakistan. Although not mentioned here at length are meat production feeds such as for beef such as pectinogenic feeds from young grasses and legumes (e. g. Spring grasses and legumes) including their involved manegerial practices for raising feedstock including giant "long fibre" Pangola grasses of the GRO variety for pectin content.

D. WATER BUFFALOES AND PROBIOTICS USES.

Are for production (meat, milk, draught) representing the tri-approach to plasmid driven boosting for animal nutrition from rumen digestion. The issue outstanding since the late 1980s has been the whole retinue of technology transfer issues entailing the usual factors of: 1) political will and administration by both parties which we believe is covered with non-GMO ethics and requires no further debate, 2) strategic holds on interests which we believe are all released as commercial interests eventually, 3) technology delivery entailing a) training, b) government funding and further subsidies afield in programming, c) capacity for commercialization and infrastructural limits in industry and d) economic factors as in the tax base, and finally 5) addressing also technological delivery in terms of sustainable supply-demand equilibrium and cyclical ability to field technology for implementation.

E. EXPERIMENTATION METHODS FOR TESTING PROBIOTICS.

For water buffalo experiments will require microbial spp. bioinformatics for studying plasmid genetics including regulons for sugars and for oligopeptides and studying the crossfeeding and self-feeding with the proteolytics spp. while at the 'baseline' the saccharolytics crossfeed and self-feed in the same manner. This in the milieu of rumen contents means either enzyme diffusion between anchored microbes to feed particles and adherence of enzymes secreted to the substrate or the medium of dissolved substrate. There is a biofilm that is inhabited by the attaching or colonizing spp. that has to be diffused through. Also, dissolved microbes also fill the liquid milieu with their own enzymes.

Plasmid are stably reproduced due to selective pressures in a very competitive environment like the rumen as long as the modifications that require ATP (cellular energy) are not overly metabolically demanding.

These are the recommendations for in vitro level studies using semi-purified substrates as the C-source + N-source + cofactors (minerals + vitamins) using a purified tri-innoculant approach (fibrolytic, proteolytic and rumen anaerobic fungal spp.) with the metabolic regulator booster, viz. PNA-B12, effectively boosting the enzymatic complement to measure the DM/OM in vitro yield in batch culture.

Further scale-up to the RUSITEC(R) device or some other simulator like Czerkawski's device using the rumen innoculant approach using mixed subpopulations with semi-processed feed substrates normally encountered in whole animal feeding experiments.

(c) D. A. Flores. 2026-2059. Skye Blue Press Corporation. Port Coquitlam, BC Canada V3B 1G3. 

Last update of this entry: September 15, 2026