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Birchland Manor Poco
C.-B.
Canada
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Description:
WATER BUFFALO is being for production (meat, milk, draught) representing the tri-approach to plasmid driven boosting for animal nutrition from rumen digestion. The issue outstanding since the late 1980s has been the whole retinue of technology transfer issues entailing the usual factors of: 1) political will and administration by both parties which we believe is covered with non-GMO ethics and requires no further debate, 2) strategic holds on interests which we believe are all released as commercial interests eventually, 3) technology delivery entailing a) training, b) government funding and further subsidies afield in programming, c) capacity for commercialization and infrastructural limits in industry and d) economic factors as in the tax base, and finally 5) addressing also technological delivery in terms of sustainable supply-demand equilibrium and cyclical ability to field technology for implementation. EXPERIMENTATION METHODS Water buffalo experiments will require microbial spp. bioinformatics for studying plasmid genetics including regulons for sugars and for oligopeptides and studying the crossfeeding and self-feeding with the proteolytics spp. while at the 'baseline' the saccharolytics crossfeed and self-feed in the same manner. This in the milieu of rumen contents means either enzyme diffusion between anchored microbes to feed particles and adherence of enzymes secreted to the substrate or the medium of dissolved substrate. There is a biofilm that is inhabited by the attaching or colonizing spp. that has to be diffused through. Also, dissolved microbes also fill the liquid milieu with their own enzymes. Plasmid are stably reproduced due to selective pressures in a very competitive environment like the rumen as long as the modifications that require ATP (cellular energy) are not overly metabolically demanding. These are the recommendations for in vitro level studies using semi-purified substrates as the C-source + N-source + cofactors (minerals + vitamins) using a purified tri-innoculant approach (fibrolytic, proteolytic and rumen anaerobic fungal spp.) with the metabolic regulator booster, viz. PNA-B12, effectively boosting the enzymatic complement to measure the DM/OM in vitro yield in batch culture. Further scale-up to the RUSITEC(R) device or some other simulator like Czerkawski's device using the rumen innoculant approach using mixed subpopulations with semi-processed feed substrates normally encountered in whole animal feeding experiments.
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Last update of this entry: September 09, 2026
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