The conventional route to making isopentenol was to adapt an enzyme structure-function molecule to adding an extra carbon (1C) with E. coli as the recombinant or modified host in their rec-plasmids. The otheer way is to randomly apply mutational change (e. g. X-ray) with selection for enzymes that do although it called to question the probabilities and ease of doing so.

 

It might be that our biomolecular intermediates pose less hazard in terms of toxicity to the host allowing higher titre of product to evolve in the rxn chamber or fermentor at lower costs per unit product. 

 

In the journey towards improvization to improve process engineering for hi-density, low-knocking alcohols as biofuels like isopentenol (5C) and isobutanol (4C) utilizing gene edited (GEditing) metabolism, in this case, from plants using E. coli (and maybe yeast down the road) as host for biofermentation using byproduct feedstocks as likely inputs and further downstream with chemical engineered processing.

 

The plant growth regulator or metabolite as starting material is called isopentenyl adenosine (IPA). The enzymes in particular won't be mentioned here for our purposes but they presumably will be cleanly spliced into the plasmids imported into the hosts in the biofermentor. 

 

 

FIG. 1 Structure of Isopentenyl Adenosine.

 

 

    BASE - NH - CH2 - CH=C - CH3         Base = Adenosine

                                      |

                                    CH3 

 

 

    

In our procedure the steps are taken as ff.:

 

1. IPA is synthesized biochemically in plants (as a growth regulator) and enzymes in that sequence are GEdited per enzyme in E. coli hosts.

 

2. Downstream chemically processed by a) H2O addition, b) H2 elimination and forming a concatenated 2 double bonded structure after some racemization, both a) and b) via catalysis and c) acid catalyzed hydrolysis and d) elimination.

 

The bi-product is adenosine (a base).