Dye-Tagged Gel Electroseparation Info Enterprises Canada

Barberry Community House
Port Coquitlam
Canada
Toll free: +011-2369836419

Phone: +011-236-983-6419
Fax: 1-604-941-9022

Description:

We are in the process of consulting future licensing manufacturers for subsidiaries.

We are gathering business intelligence near Boston, MA USA that our so-called "Digitel Laser Device" can be developed in the USA (with Raccoon InfoSyse as the Canadian & Philippine manufacturing subsidiaries).

We will contact candidates to be licensed exclusively with use of our dye-tagged denaturing electrophoretic protocol using microcapillary resin columns which beats the competition for fast turn around of our column or line runs and ease of use otherwise not available and will facilitate faster multiple runs for high-throughput proteomic analysis.

Additionally it is requisite that the electrophoretic mechanism be "pulsed" to define greater resolution and finer discrete bands and reading capacity through the laser reader. 

In the news: (The reader is invited to research the Internet for more of the same news reported here.)

Thermo-Fisher Scientific Ltd. (R) demonstrated in ads on social media microreplicated arranged cassettes in semi-automated, rapid digitized or electronic run and read/processed data recording output. Breathtaking to say the least and promising as a partner for mini-protein reader proposals from “SkyeBlue” for their DTGE methodology subject to the need to ascertain integration of output bands vs background stacked (and which may unstack) at the critical micellar concentration (CMC) of the detergent in solution in media in the microcapillary.

Bio-Rad® and their “Substrate-Level” Chromophore Tagged (“Dyed”) Protein PAGE with Mini-Protein Reader.

This is the competition to our technological breakthrough but is not prep ready for ID and quantitation for the protein bands analyses in case recovery is desired from the sample. There is also the question of labour and time constraints for prepping the microcapillary column ff. by analyses and data processing is complete. 

Amersham(R) in Australia was also approached earlier as a first choice for developing the dye-tagging reagent and further proposals to finish its commercialization with a digitized reader that we decided to provide licensing from exclusive rights from "SkyeBlue" of Pt-Coquitlam, BC Canada with half rights in the world to be licensed to our associated Canadian independent subsidiary through a relational connection.

We have found that our electrophoretic technology will change denaturing PAGE methodology due to its facility in the laboratory including semi-automated prep work, an automated run for loading, delivery through, reading and quantitation or output data collection of the digital device.

Computational analytics will predominate with proteomics which will be an expansive field to grow with playing in, for e. g., QC with stem cell-based clean meat fermentation, pharma, fine-biochemicals, hormones, enzymes and nutraceuticals or nutritionals.

SKYEVIEW: 2025-04-27. NIH earlier reported for us although not as in a pro-bono consultancy basis that they agreed that the molecular approach to DTGE separation functions as described requires consensual support and agreement for it to be invested into taking it a step closer to reality as a viable alternative to the much used denaturing gel electrophoretic technique used in research today. Will it take political will from Washington DC alone, together with support and policy leads for its development and establishment in biomedical research and its related fields rather than simply commercially launched with reagent kit manufacturing and a matched digitel reader laser-based device that some companies can be contracted for due to their capabilities in manufacturing, for e. g. a conglomerate based in Canada like Thermo-Fisher Scientific Ltd., which to our view would be daunting from 'square one' unless agreed upon through industrial agreements and political funding rather than from a commercially promotional sales strategy. 

SKYEVIEW: 2025-12-04. On the wire we have gathered further news from Thermo-Fisher Scientific Ltd. from N. J. USA that indeed their modern electroseparating apparatus with microcapillary hi throughput and hi spped capacity functions with the ff. hindrances: 1) the SDS detergent reagent cannot recover the original de novo analyte or product, 2) to ID the successive analytes ninhydrin reagent is added to the proteins in a double infusement and are measured with optometric consistency and 3) are recovered as such after reloading samples and gathering coherency in their relative mobilities, quite a tedious complicated task vs. our much more advance technique with a dye tag that is removable by separation (it is diffusable) in column and can be sequentially detected through the dye and the de novo product recovered at such a high capacity rate and then micro sequenced with an amino acid analyzer and used for DNA/mRNA expression and a fermentor for sample prepartion to study primary sequencing, active site localization, mechanistic studies, with mathematical kinetics in tow, and finally definitive microstructural chracterizations and secondary (beta sheet, alpha helix), and tertiary subunit interactions, i. e. regulatory control and with respect to the active site through definitive X-ray diffraction. 

FIG. 2.  Using Hydrazine (NH2-NH2) Chemistry variate the chemical structure of our molecular reagent with only 1 methylene group between the Azo unsaturation and the Sulfonate group with a total of 12-C length in its side-chain. 

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1. Starting material is Undecanoic Acid.

    CH3(CH)n=9-COOH 

2. Reduction of the Carboxyllic acid to the Aldehyde.

   CH3(CH2)n=9-CHO

3. Alpha-Bromination.

   CH3-(CH2)n=8-CH(Br)-CHO 

4. Adduct formation with the Hydrazide to the Ene-Azo-Amine.

    CH3-(CH2)n=8-CH=CH-N=N-CH2-NH2 

     (Note: Bi-product of the Ene-Amine-Hydrazide.) 

5. Formation of the Ene-Azo-2-Methyl-thio-pyridinium Nitrate via 2-Thiomethylpyrdinium      Nitrate.

    CH3-(CH2)n=8-CH=CH-N=N-CH2-2-Methylthiopyridinium(+)NO3(-) 

6. Sulfate Formation with Cl(SO3)H

    CH3(CH2)n=8-CH=CH-N=N-CH2-ONO2 

7.  Final Product:  Ene-Azo-Sulfate (Dye-detergent)

     CH3(CH)n=8-CH=CH-N=N-CH2-(SO3)-H  

SKYEVIEW: In mammalian species dairy cows have been found to be responsive to calf growth and development via HIS protected supplements unless injected directly and is the most likely limiting amino acid (cf. A. N. Hristov) for milk synthesis in the cow's udder. Most likely we will want to pursue the same replicated research for ultra-bred cows (even more elite than HI-bred spp.) that have been boosted further for genetically regulatable organismal (cf. non-GMO) or termed GRO boosted cows with TFs boosting the operons probably for bGRH/bGH. 

(c) 2025-2059. D. A. Flores. SKYE BLUE (SB) INTERNET. Port Coquitlam. BC. Canada V3B 1G3          

Last update of this entry: December 05, 2025