Dye Tagged (DT) Capillary Electrophoresis (CE) (Gel and non-Gel) (G and NG)Info Enterprises Canada

Creating Employment for Job and Economic Growth in the Philippines
Colombie-Britannique (C.-B.) V3B 1G3
Canada
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Description:

We are in the process of consulting future licensing manufacturers for subsidiaries.

We are gathering business intelligence in the USA for our "Digitel Laser Device" to be developed with Canadian & Philippine manufacturing subsidiaries. A partner in Canada with exclusive rights to use the dye tag is planned and another in DGT City (NIR), PHILIPPINES.

We will contact candidates to be licensed exclusively with use of our dye-tagged denaturing electrophoretic protocol using microcapillary non-gel columns for hi-throughput, hi-speed proteomic and peptide analysis as well as gel electrophoresis for more larger samples more limited runs which beats the competition for fast turn around of our column runs with faster, ease of use not available to conventional dyed in gel protocols.

The denatured analytes with attached dye-tags will be isoelectric focused by an electric field gradient along the cartridge of loaded sample multiple non-gel capillaries or larger sample gel columns, separation being based on size/MW and net charge for each protein type analyte. 

The feature of running parallel samples (x2), one with denaturing dye-tag and the other with no dye added will allow prep recovery of the sample in question of interest in this type of analysis.

In the long term of interest to our particular independently run Philippine-based subsidiary in Dumaguete (DGT) City (NIR), the PHIL. is to use our digitel reader and for marketing and capitalization of company facilities with exclusive licensing for the dye tag (DT) reagent product which also will be manufactured multinationally and with licensed establish chemical cos. especially in the U.S. of A.

The competition we expect or believe are going to field similar analytical machines with laser-driven reading capability without a dye-tag and will rely on less quantitave methods to read analytical peaks and using a universal method of mechanically collecting aliquots that are resolvable by titre and displacement. But without quantitative capability it is less elegant and automated for routine quantitation for QC analysis runs as in food sciences & technology industry protocols for such food-grade analytes creating extra market share for our product.

In both the competition's device or reader conventional protocol and our own DT with protocol the reader will have to be cost-effective to compete successfully in the very fast-growing and lucrative bioinformatics field and metabolomics field which is just beginning with proteomics and its genomics fields.

There has been mention of prioritizing the bioinformatics-led development of the Direct Applied RNA-Based Regulatory (DARR) protocols led by the inventions from research at the U. of Warsaw, Poland by J. Trylska's and group using the fine biologic PNA-B12 with Vit B12 as the carrier through cellular introduction and in our case a new candidate proprietarily protected here on this website called PNA-K2 with Vit K2 as the carrier and with linker/spacer as the carrier attached to the regulatory moiety of the fine biologic in its macromolecular structure.  At Skye Blue, the author and believes that A-prioritization should occur with the development of applications with problems for forage cropping, microbial probiotics, microbial feed additive prebiotics and large animal metabolism in livestock production models, the latter which is believed to be extensive with animal nutrition, health and animal welfare. 

In the news: [The reader is invited to research the Internet for more of the same news reported here. Including recent reports of what is called the Maurice Flex Cartridges(R) by Bio-techne(TM)].

Our overall Corporate "game plan" for the Denaturing Gel Tagged Electrofocused capillary electrophoresis (DGT E-CE) technique to displace and compete with the SDS PAGE protocol includes a dual strategy between chemical reagent mfging and digitel machine reader which is laser based mfging. With a contact in the metro capital area of Manila, the Philippines we reach out with financial services and legal services from Skye Blue Corporation based in Canada. It is understood that a CDMO for reagent contract mfging and CDMO for contract mfging of the biomed. device, the device paralleling Bio-techne's(TM) core isoelectric focusing technology with the Maurice Flex cartridges(R) to be set up by our present Principal and Owner first based in Manila and then in Tokyo, Japan; Toronto, Canada; MA USA; Mexico City, Mexico; Abu Dhabi; with licensed machines if agreeable to Thermo-Fisher (MA, USA), Bio-Rad (USA) and Amersham (AU). As a corp. world-wide we strive for ownership (decision-making) and shareholder control including lifetime ownership of the Detergent-Dye tagging reagent for analyte as licensed to partners through the eventual CEO of Skye Blue Corporation or whichever name it call signs by outside our other umbrella, the Skye Blue Group, in Canada. The CEO on internal agreement will obtain and underwrite financial loan services and legal services and garner a 40::60 % cut in shareholdings and overall decision-making hold in day-to-day company affairs. These will be also offered via IPOs to Venture capitalist investors.  Any initial stock offerings will be announced by the CEO and his Board Committee members. We are happy at this juncture to announce a Venture Capital fundraising agency in FL USA who can offer a private investor's funds (we are looking at $50M) from Canada, possibly, and another tranch ($50M) from the same investors through the Venture Capital fundraising agency for the first subsidiary for device mfging based in MA USA and eventually to branch out to Mexico City, Mexico. There may be also subsidiary branchings via Manila and Tokyo and to Manila and Abu Dhabi.  

Thermo-Fisher Scientific Ltd.(R) USA could be an interested co-licensee in this new endeavor in electroseparation. Bio-Rad(R) USA is also another potential co-licensee with their “Substrate-Level” Chromophore Tagged (“Dyed”) Protein PAGE and Mini-Protein Reader in the competition. Note this is also a viable alternative to our method. Amersham(R) Australia was also approached earlier as a first choice for developing the dye-tag reagent. We will try them again in Australia with further negotiations.

It is believed that parties in Silicon Valley (Palo Alto CA USA) will be currently investing in the Far East for AI-upgrading of the workforce in the Philippines with expanded Data Centre infrastructuring, and present issues for land use planning and utilities (e.g. water, solar energy) and even hard mineral commodities for mining and environmental control and protection the Philippines shows some abundance in Cu, Ni, Cd. 

We have found that our electrophoretic technology will change denaturing SDS-PAGE due to its facility in the laboratory including semi-automated prep work. We are also proposing here isoelectric non-gel prep separation as another option for use in analysis.

Computational analytics will predominate with proteomics with the [LMW]-proteome from secretagogue body fluids or blood plasma, clean fermentation sampling for food-related products, drug discovery with biopharma, endo and neurohormones, enzymes, nutraceuticals, antibodies for immuno analysis and the multiplicity of biomarkered research for metabolomics. Metabolomics is just intiating agrocrop, livestock production and health research growth or expansion at this time. 

CDMOs are key to seeing technology succeed into commercialization although we are just initializing Venture Capital investing with hefty financial fee payments and in contact with Ekting Fundraising Services of Palm Beach FL USA. There is already word on the street in the metro-Manila the Philippines about possible CDMO entities available for long-term contracting by which obtaining services of a research chemist(s) are realized through possible contacts to the Dilliman and UPLB campus and other leading universities. It should be announced that our own researcher has documented with the science literature one (Pathway B) route or scheme and a second one (Pathway A) remaining undocumented but in theory correct, to be completed at our CDMO's library.

The lab bench research chemist will operate documenting the frame work of verifying parameters of the desired endproduct or reagent for its: MW (calculated), reaction to completion via TLC, absorption (lambda max, epsilon), m.p., PMR spect., IR spect., mass spec., and total % yield for the economic parameter to select and approve the viably commercial synthetic route(s). 

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FIG. 2  Practical Schema Using Hydrazine (NH2-NH2) Chemistry to Variate the Chemical Structure of Our Molecular Reagent with Only 1 Methylene Group Between the Azo Unsaturation and the Sulfonate Group with a Total of 12-C Length in its Side-chain. 

1. Starting material is Undecanoic Acid.

    CH3(CH)n=9-COOH 

2. Reduction of the Carboxyllic acid to the Aldehyde.

   CH3(CH2)n=9-CHO

3. Alpha-Bromination.

   CH3-(CH2)n=8-CH(Br)-CHO 

4. Adduct formation with the Hydrazide to the Ene-Azo-Amine.

    CH3-(CH2)n=8-CH=CH-N=N-CH2-NH2 

     (Note: Bi-product of the Ene-Amine-Hydrazide.) 

5. Formation of the Ene-Azo-2-Methyl-thio-pyridinium Nitrate via 2-Thiomethylpyrdinium Nitrate.

    CH3-(CH2)n=8-CH=CH-N=N-CH2-2-Methylthiopyridinium(+)NO3(-) 

6. Sulfate Formation with Cl(SO3)H

    CH3(CH2)n=8-CH=CH-N=N-CH2-ONO2 

PRODUCT:  Ene-Azo-Sulfate (Dye-detergent)

     CH3(CH)n=8-CH=CH-N=N-CH2-(SO3)-H  

(c) 2026-2059. D. A. Flores. SKYE BLUE (SB) INTERNET. Port Coquitlam. BC. Canada V3B 1G3          

Last update of this entry: August 07, 2026