Proteome Factory AG

Magnusstr.11
D-12489 Berlin
Germany

Phone: ++49 30 20616265
Fax: ++49 30 20616267
E-Mail: This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
Please notice: This entry hasn't been updated by the submitting company for more than 2 years. It could be possible that this company doesn't longer exists.

Description:

Company objectives
Proteome Factory AG offers contract research and fee-for-service analyses in the field of protein analysis, analysis and characterization of biosimilars and therapeutic/recombinant proteins, proteome analysis (proteomics), protein identification characterization, protein sequencing (N-terminal, internal sequencing and sequencing of complete proteins).

Proteome Factory AG offers and develops new technologies for high throughput differential proteome analysis.

Contract research service / fee-for-service:

- Proteomics (gel-based and gel-free approaches):

• Protein analysis and characterization of therapeutic and recombinant proteins and peptides
• Biomarker discovery and identification
• Biomarker profile discovery and identification
• Protein and peptide target discovery and identification
• Identification of regulated proteins and protein species
• Optimization of fermentation processes
• Optimization of plant breeding
• Pharmaco proteomics
• Identification of vaccine candidates
• Immuo proteomics
• Phospho proteomics
• Membrane proteomics

Technology plattform:
- high resolution mass spectrometry / nanoLC-MS

- high resolution 2D electrophoresis 23x30cm, 40x30 cm (separation of up to 10000 protein spots)
- 2DLC-ESI-MSMS (label free, ITRAQ, MeCAT)
- depletion of high abundant plasma/CSF proteins by affinity depletion
- prefractionation (e.g. phospho proteins, membrane proteins)
- Blue native PAGE / SDS-PAGE etc.

- Identification, sequencing and characterization of proteins, biosimilars and biobetters

• protein ID by nanoLC-ESI-MSMS of individual or low complex protein samples
• protein ID by MALDI-TOFTOF-MS of individual proteins
• MudPIT (multi dimensional protein identification technique) of complex protein samples
• analysis of phosphorylation sites
• analysis of glycosilation sites
• analysis of PTMs (post-translational modification)
• analysis of deamidation and oxidation, lysine clipping etc.
• AA sequening by mass spectrometry

- Protein sequencing

• N-terminal protein sequencing
• N-terminal peptide sequencing
• Internal protein sequencing
• Sequencing of complete proteins by combination of mass spectrometry, N-terminal sequencing and HPLC fractionation of proteolytic peptides generated by protein cleavage with several proteases/chemicals

- Analysis and Quality Control of protein and peptide samples etc.

• Analysis, identification and characterization of products and batches
• Analysis of the composition of complex protein mixtures (protein display)
• Comparison of the composition of complex protein mixtures (e.g. product comparison)
• Analysis of protein and peptide stability
• Analysis of the pharmacokinetic of protein and peptides, e.g. antibodies
• Assistance for optimisation of fermentation and production by protein analysis

Products:

• MeCAT (metal coded tagging) multiplex kit for proteomics and absolute quantification of proteins and peptides by ICP-MS
• Breakable DPAGE kit for 2DE and SDS-PAGE (DPAGE allows the dissolving of PAGE after protein separation)
• FireSilver kit - high sensitive silver staining kit, highly compatible with protein ID by MS
• FireRuth protein fluorescent staining solution, ready-to-use
• FirePhospho-protein enrichment kit (coming soon)

• spotXpress- HTS spotpicking robot (up to 800 spot/hour)
• spotDigest 96 - sample preparation robot for protein identification of protein spots cut by spotXpress

Last update of this entry: September 28, 2021