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Author Topic:   Primers
posted 05-09-2011 05:22 AM     Click Here to See the Profile for Rafid   Click Here to Email Rafid     Edit/Delete Message Reply w/Quote
Dear all Hum-Molgen forum members,
Currently I am doing PCR for certain gene polymorphisms. CYP3A4*5 (653C>G)is one of the gene polymorphisms that I am targeting, I managed to design primers for this polymorphism (attached down with the message) with a PCR product size of 366bp. Blasting the primers in pubmed resulted in many products, among which the one that I am targeting.
Please can I get your kind help and opinion to confirm the specificity and reliability that these primers will give the wanted product.

Thanking you in advance.


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