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 DIAGnostics - Clinical Research (professional requests)
 nonsence or frameshift mutation-detection
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| Author | Topic: nonsence or frameshift mutation-detection |
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maria roque Member |
posted 06-03-2003 02:39 PM
   
I am trying to check the presence of stopcodons in cancer-related genes in families with hereditary cancers. We are thinking in making a plasmid with color reporter genes to transform bacterias. Does anyone have experience in this? thanks mroque@fcm.uncu.edu.ar IP: 200.69.149.70 |
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Jonathan_Liu Member |
posted 08-22-2003 07:01 PM
We suggest use the DNA sequence method, the golden standard for mutation discovery and mutation diagnostics. We have software tool, Mutation Surveyor, to find such kind of mutations with DNA sequence traces. In our report, the stop codon and frame shifts (homozygous and heterozygous insertion and deletion) are specially designed for this study. If you are interested, please response to this email and I will send you a CD of software. You may visit our web at www.softgenetics.com to down load software as well. ChangSheng Jonathan Liu, Ph.D. IP: 68.168.163.27 |
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jkennedy Member |
posted 09-10-2003 10:49 AM
To detect stop codons specifically, the PTT (Protein Truncation Test) will probably be the quickest and most specific for this type of mutation. It is used widely in molecular diagnostic labs, but is being superseded by direct DNA sequencing which will, of course, detect the majority of sequence changes. IP: 193.60.159.61 |
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