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  HiLyte™ Your Antibodies Using One Universal Reagent

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Author Topic:   HiLyte™ Your Antibodies Using One Universal Reagent
posted 01-29-2015 12:07 AM     Click Here to See the Profile for anaspec   Click Here to Email anaspec     Edit/Delete Message Reply w/Quote
AnaSpec is pleased to announce the release of a product line of recombinant Protein A, conjugated with HiLyte™ Fluor dyes or Biotin. This universal reagent can be used to detect antibodies from different species, such as human, rabbit, mouse, rat and others. Protein A is available as labeled with HiLyte™ Fluor 488 (Ex/Em = 499/523 nm), HiLyte™ Fluor 555 (Ex/Em = 553/568 nm), HiLyte™ Fluor 647 (Ex/Em = 649/674 nm), HiLyte™ Fluor 680 (Ex/Em = 678/699 nm), and HiLyte™ Fluor 750 (Ex/Em = 754/778 nm), or biotin. The large panel of emission wavelength HiLyte™ labeled Protein A enables the visualization of different target proteins within one species. Additionally, HiLyte™ Fluor dyes exhibit brighter signal compared to classic dyes (FITC, FAM, TAMRA, etc.).

Protein A is a non-glycosylated cell wall protein of Staphylococcus aureus that can bind the Fc part of immunoglobulin molecule of different species with strong affinity (1-4). Protein A consists of five IgG binding domains (A, B, C, D, and E), each approximately 60 amino acids long with no cysteines present and two S. aureus cell membrane binding domains - X and M (3).

AnaSpec’s recombinant Protein A consists of only IgG binding domains and was expressed in E.coli. Its apparent molecular weight is approximately 30,000 Da compared to the most reported 42,000 Da for the native Protein A that is found in S. aureus.

Recombinant Protein A conjugated with HiLyte™ Fluor 488 can be used for immunoglobulin detection in IHC and IF and for assay development. Biotinylated Protein A can be used in the aforementioned applications, and for western blotting.

For more information, please visit: http://www.anaspec.com/products/promotions.asp?id=133&col=1&row=1

1. Björk, I. Eur J Biochem 29 (1972):579-584.
2. Sjödahl, J. Eur J Biochem 78 (1977): 471-490.
3. Schröder, A. K. et al. Immunol 57 (1985): 305-309.
4. Rosenberg, I.M. Protein Analysis and Purification, Birkhäuser, 2005: 41-47.

AnaSpec, EGT Group
AnaSpec, EGT Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and Takyon™ qPCR MasterMixes. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening (HTS) and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.

For more information visit: www.anaspec.com


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