posted 10-30-2013 10:35 PM            

Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 °C, making it the protease of choice for fusion proteins or tags removal (1-3).

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References
1. Fang, L. et al. Prot Expr Pur 51, 102-109 (2006).
2. Blommel, PG. et al. Prot Expr Pur 60, 1-16 (2007).
3. Lucast, LJ. et al. Biotechniques 30 (3), 544 (2001).

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