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  AnaSpec Introduces SensoLyte® 520 TEV (Tobacco Etch Virus) Protease Assay Kit

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Author Topic:   AnaSpec Introduces SensoLyte® 520 TEV (Tobacco Etch Virus) Protease Assay Kit
anaspec
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posted 10-30-2013 10:35 PM     Click Here to See the Profile for anaspec   Click Here to Email anaspec     Edit/Delete Message Reply w/Quote
AnaSpec is pleased to announce the recent release of the SensoLyte® 520 TEV Protease Assay Kit. This kit uses a TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL™ 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL™ 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly proportional to the TEV protease quantity/activity.

Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 °C, making it the protease of choice for fusion proteins or tags removal (1-3).

For more information, please visit http://www.anaspec.com/products/product.asp?id=57834

References
1. Fang, L. et al. Prot Expr Pur 51, 102-109 (2006).
2. Blommel, PG. et al. Prot Expr Pur 60, 1-16 (2007).
3. Lucast, LJ. et al. Biotechniques 30 (3), 544 (2001).


AnaSpec, EGT Group
AnaSpec, EGT Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and Takyon™ qPCR MasterMixes. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.

For more information visit www.anaspec.com

IP: 12.169.96.11

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