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Author Topic:   Application of Restriction Enzyme
wareturs
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posted 06-12-2013 05:28 AM     Click Here to See the Profile for wareturs     Edit/Delete Message Reply w/Quote
Restriction endonucleases can be identified specific sequences of DNA and the recognition site of cutting around the double-stranded DNA or of a class of enzymes, called restriction enzymes. The structure of the restriction enzyme, cofactor requirements cut position and the mode of action of restriction enzyme can be divided into three types, namely a first type (Type I), a second type (Type II), and a third type (Type III ). ¢ñ type restriction endonuclease catalyzed both host DNA methylation, and non-methylated DNA catalyzed hydrolysis; the restriction endonuclease type ¢ò only unmethylated DNA catalyzed hydrolysis. Type III restriction endonuclease recognition both modified and cutting effect.

DNA genome physical map for the formation; gene mapping and gene isolation; DNA molecule nucleotide sequence analysis; comparing related DNA molecules and genetic engineering.

Restriction endonucleases are produced by bacteria, and its physiological significance is to improve its defense capabilities.

Restriction enzyme generally does not cut the DNA molecule itself, only the cutting exogenous DNA.

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