home genetic news bioinformatics biotechnology literature journals ethics positions events sitemap

UBBFriend: Email This Page to Someone!
  Biotechnical requests and sources
  AnaSpec Introduces SensoLyteģ ThT A‚42 & A‚40 Aggregation kits

Post New Topic  Post A Reply
profile | register | preferences | faq | search

next newest topic | next oldest topic
Author Topic:   AnaSpec Introduces SensoLyteģ ThT A‚42 & A‚40 Aggregation kits
posted 11-07-2012 08:22 PM     Click Here to See the Profile for anaspec   Click Here to Email anaspec     Edit/Delete Message Reply w/Quote
SensoLyteģ ThT Aß40 and Aß42 Aggregation kits, using the fluorescent dye Thioflavin T (ThT), provide a convenient and standard method to measure Aß40 and Aß42 aggregation, respectively. In each of the kits, Aß40 or Aß42 peptide is pretreated to ensure it is in a monomeric state. Included in the kits is an optimized fibrillation buffer and two known inhibitors as controls. The assay is based on the property of ThT dye in which fluorescence (Ex/Em=440/484 nm) increase is proportional to the amount of dye bound to aggregated Aß peptides.

Alzheimer's disease (AD), the most common cause of dementia, is characterized by the presence of senile plaques and neurofibrillary tangles, surrounded by damaged neurons. Beta-Amyloid (Aß peptides, Aß40 (1-40) and Aß42 (1-42), were found to be a major component of the above plaques. Many studies suggest that these peptides can form toxic oligomers and fibrils under physiological conditions and rapidly aggregate. Since Aß aggregation is evidently an essential event in the pathogenesis of AD, a reliable assay is important to study Aß fibrillation kinetics and for screening Aß aggregation inhibitors.
For more information, please visit http://www.anaspec.com/products/promotions.asp?col=1&row=1

1. Hellstrand E., et. al., ACS Chem. Neurosci., (2010) 1: 13-18.
2. Karafin A., et. al., MEDIMOND, (2009) 11-15: 255-259.
3. Hudson S. A., et. al., FEBS J., (2009) 276: 5960-5972.
4. Liu R., et. al., Neurobiol. of Disease, (2005) 20: 74-81.
5. Kudva Y. C., et. al., Biochem. J., (1998) 331: 809-813.


All times are ET (US)

next newest topic | next oldest topic

Administrative Options: Close Topic | Archive/Move | Delete Topic
Post New Topic  Post A Reply
Hop to:

Contact Us | HUM-MOLGEN

Copyright by HUM-MOLGEN 1995-2017

Powered by: Ultimate Bulletin Board, Version 5.44a
© Infopop Corporation (formerly Madrona Park, Inc.), 1998 - 2000.