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  AnaSpec Offers Double-Dye Oligo Synthesis Service

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Author Topic:   AnaSpec Offers Double-Dye Oligo Synthesis Service
posted 12-20-2010 09:20 PM     Click Here to See the Profile for anaspec   Click Here to Email anaspec     Edit/Delete Message Reply w/Quote
AnaSpec, Eurogentec Group is pleased to highlight our Double-Dye Oligo Synthesis Service. With over 20 years expertise of oligonucleotide synthesis, and Eurogentec production facilities in San Diego, California and Liege, Belgium, AnaSpec now offers the largest number of fluorescent reporters and quenchers available in the industry. Furthermore, for applications such as genotyping or gene expression profiling, requiring small amounts of several different probes, we have developed a high-throughput platform capable of producing high quality small scale Real-Time qPCR Double-Dye probes. All Double-Dye Probes are HPLC purified. They are quality controlled by analytical HPLC and MALDI-TOF Mass Spectrometry and are provided lyophilized in individual tubes.

Double-Dye Oligonucleotides have a fluorescent reporter dye and a quencher at their 5’ and 3’ ends. These oligos are designed to anneal between the upstream and downstream PCR primers. The higher Tm of the Double-Dye Oligonucleotide probe compared to the Tm of the upstream primer ensures that the primer is already being extended while the Double-Dye Oligonucleotide probe is not yet annealed. The optimal recommended length of Double-Dye Oligonucleotide probes is 25 bases, and maximum length is 30 bases. For extra long Double-Dye Oligonucleotides probes, quenchers like Black Hole Quencher™, Deep Dark Quencher I and TAMRA can be coupled internally.


About AnaSpec, Eurogentec Group

Since 1993, AnaSpec, Inc is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. As a subsidiary of Eurogentec, AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, oligonucleotides, and qPCRs. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.

For more information visit www.anaspec.com


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