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  AnaSpecs Offers Dyes for labeling Amine- and Thiol-Modified Oligos

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Author Topic:   AnaSpecs Offers Dyes for labeling Amine- and Thiol-Modified Oligos
posted 12-09-2010 07:35 PM     Click Here to See the Profile for anaspec   Click Here to Email anaspec     Edit/Delete Message Reply w/Quote
AnaSpec is pleased to highlight our own proprietary HiLyte Fluor dyes for labeling amine and thiol modified oligonucleotides from AnaSpec’s wide collection of amine and thiol reactive 6-isomeric dyes of fluoresceins and rhodamines. Fluorescent dye-labeled oligos are widely used as primers for sequencing and PCR reactions, and also as probes for fluorescence in situ hybridization (FISH). Double-labeled oligos are used to produce fluorescence resonance energy transfer (FRET) for a number of applications such as real-time PCR assays and automated DNA sequencing.

Synthetic oligos can be modified with amine or thiol groups at their 5’-end, 3’-end or internally. These amine and thiol incorporated oligos can then be labeled with amine and thiol reactive fluorophores, respectively. N-hydroxysuccinimidyl esters (SE) and isothiocyanates of fluorescein and rhodamine derivatives are used for labeling amine-modified oligos. Maleimides and iodoacetamides of fluorescein and rhodamine derivatives are used for labeling thiol-modified oligos.

AnaSpec’s proprietary HiLyte Fluor™ dyes are available in the SE and maleimide forms. These dyes have fluorescence emission wavelengths spanning the full visible and near infrared (NIR) spectrum. HiLyte Fluor™ dyes have properties that are superior to conventional dyes, with high fluorescence quantum yields, large extinction coefficients, good photostability, low pH sensitivity and improved water solubility.


About AnaSpec

AnaSpec is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and antibodies.
AnaSpec recently joined forces with Eurogentec to provide an even broader spectrum of products and services to serve the worldwide life science community.

For more information visit www.anaspec.com

About Eurogentec

Eurogentec is a leading global supplier of innovative reagents, kits, specialty products and custom services to scientists in the life science, biotechnology, pharmaceutical and diagnostic markets. Eurogentec provides a wide range of expertize in small- and large-scale DNA, RNA, PCR and qPCR kits, peptide synthesis and antibody supply for research applications. Our ISO13485:2003-certified manufacturing facilities in Belgium provides a wide range of high value oligonucleotide-based components for diagnostic and therapeutic applications. Eurogentec’s Belgium manufacturing facility is complemented by additional production facilities in North America and Japan. Eurogentec is also an experienced Contract Manufacturing Organization (CMO) for Biopharmaceuticals, operating a full-service, state-of-the-art GMP facility in Belgium.
Eurogentec is a privately held company headquartered in Liège, Belgium, with subsidiaries in North America, France, Germany, the UK, the Netherlands and Switzerland and has additional production facilities in North America, Japan and Singapore. Eurogentec employs 400+ people globally.

For more information visit www.eurogentec.com


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