posted 07-21-2009 08:17 PM            

b-galactosidase, a widely used reporter enzyme, is used in the study of gene expression, protein-protein interactions,1 and normalization of transfection efficiency.2 â-galactosidase, enzyme encoded by the lacZ gene of E. coli, catalyzes the hydrolysis of â-galactosides into monosaccharides.

AnaSpec is pleased to announce the release of 3 new assay kits for measuring â-galactosidase activity – the SensoLyte® MUG â-Galactosidase Assay Kit (Ex/Em= 360/460 nm), SensoLyte® FDG â-Galactosidase Assay Kit (Ex/Em= 490/520 nm), and SensoLyte® ONPG â-Galactosidase Assay Kit (Abs=420 nm). One advantage of using a fluorogenic substrate, as is the case in the first two assays, is that it is possible to monitor expression in individual cells as well as analyze the heterogeneity of expression in cell populations. 3 Another major advantage is that fluorimetric assays are several times more sensitive than colorimetric assays.

References
1. Rossi, F. et al. PNAS 94, 8405 (1997).
2. Thompson CD et al. Biotechniques 27, 824 (1999).
3. Plovins, A. et al. Appl. Environ. Microbiol. 60, 4638 (1994).

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