posted 07-10-2009 12:20 AM            

In response to the growing needs of the research community for zebrafish specific antibodies, peptides and other products, AnaSpec has launched the exciting new Z-Fish™ series of antibodies. Z-Fish™ antibodies are raised strictly from peptide antigens derived from zebrafish protein sequences, i.e. 100% zebrafish sequence homology. Leveraging AnaSpec’s expertise in peptide technology, the antigens used in raising these antibodies include non-phosphorylated and phosphorylated peptides. Z-Fish™ antibodies are provided in a 150 ěl size, which is optimal for 15 mini gels.

Zebrafish has been used as a developmental model in elucidating the role of MMPs during embryogenesis/development. Zhang, J. et al. isolated and characterized zMMP-2 (which shows >80% identity and >90% similarity to its human counterpart);1 Yoong, et al, zMMP-9; 2 and Hildegass, JM. et al. zMMP-13 (52% identity with human or mouse);3 among others. AnaSpec is pleased to introduce a line of Z-Fish™ antibodies for MMP-2, 9, 11, 13, 14, 17 and 24. These antibodies were raised from sequences derived from the hinge region, internal or C-terminal sequence. They have been verified in ELISA and western blots using zebrafish lysates (see western blots below).

Soon-to-be-released Z-Fish™ antibodies include antibodies for MMP-18 (IN), MMP-18 (hinge), MMP-21 (IN), and MMP-28 (hinge).

References:
1. Zhang, J. et al. Dev. Genes Evol. 213, 456 (2004).
2. Yoong, S. et al. Genes Expr. Patterns 7, 39 (2007).
3. Hillegass, JM. et al. Toxicol. Sci. 100, 168 (2007).

About AnaSpec

AnaSpec is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and combinatorial chemistry.

For more information visit www.anaspec.com

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