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  AnaSpec Introduces Industry’s First FRET-Based Thrombin Assay Kit

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Author Topic:   AnaSpec Introduces Industry’s First FRET-Based Thrombin Assay Kit
anaspec
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posted 05-28-2009 07:17 PM     Click Here to See the Profile for anaspec   Click Here to Email anaspec     Edit/Delete Message Reply w/Quote
San Jose, CA – May 26, 2009

AnaSpec has announced the launch of the industry’s first FRET-based thrombin assay – the SensoLyteŇ 520 Thrombin Activity Assay Kit.

Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation.1 Thrombin also influences a number of normal and pathological processes, including inflammation,2 tissue repair,3 embryogenesis,4 angiogenesis,5 and tumor invasion.6

The SensoLyteŇ 520 Thrombin Activity Assay Kit kit employs a novel internally quenched 5-FAM/QXLÔ 520 FRET substrate (Figure 1). Thrombin cleaves the FRET substrate into two separate fragments resulting in release of the fluorescence of 5-FAM, a green dye. Increase of fluorescence can be monitored at the commonly used excitation and emission wavelengths of 490 nm and 520 nm, respectively. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds, resulting in a high signal to background ratio. Figure 2 shows the inhibition data.

AnaSpec also offers a shorter wavelength thrombin assay, which employs an AFC (7-amido-4-trifluoromethylcoumarin) labeled substrate, the SensoLyteŇ AFC Thrombin Activity Assay Kit.

References
1. Crawley, JT. et al. J. Thromb. Hemost. 5, 95(2007).
2. Croce, K. et al. Curr. Opin. Hematol. 14, 55 (2007).
3. Artuc, M. et al. Exp. Dermatol. 15, 523 (2006).
4. Griffin, CT. et al. Science 293, 1666 (2001).
5. Maragoudakis, ME. et al. Biochem. Soc. Trans. 30, 173 (2002).
6. Snyder, KM. et al. Semin. Thromb. Hemost. 34, 734 (2008).


About AnaSpec

AnaSpec is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and combinatorial chemistry.

For more information visit www.anaspec.com

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