posted 04-10-2009 11:02 PM            

Peroxidases are a group of heme­containing enzymes that catalyze oxidation-reduction reactions. Hydrogen peroxide (H2O2) or lipid peroxides are the substrates used by peroxidases for a number of oxidative reactions. Peroxidases are found in bacteria, fungi, plants and animals. Cellular peroxidases play an important role in protecting cell from oxidative injury. Horseradish peroxidase (HRP) is extensively used in ELISA and IHC as a reporter molecule when conjugated with secondary detection reagents.

AnaSpec has introduced two new luminescent read-out assay kits for determining peroxidase activity – the SensoLyteŇ Luminescent Peroxidase Assay Kit and the SensoLyteŇ Luminescent Peroxidase ELISA Assay Kit. The former detects peroxidase activity in biological samples and in ELISA with HRP-conjugates while the latter detects HRP conjugated with antibody or streptavidin in ELISA. In both assays, the chemiluminescent substrate luminol is oxidized by peroxide and creates an excited state intermediate product that decays to a lower energy state by releasing photons of light.

The kits provide ample materials to perform 500 assays in a 96-well format. The protocol can readily be modified to run assays in a 384-well format.

About AnaSpec

AnaSpec is a leading provider of integrated proteomics solutions to the world’s largest biotech, pharmaceutical, and academic research institutions. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents, and combinatorial chemistry.

For more information visit www.anaspec.com