posted 04-22-2008 12:21 AM            

Utilizing AnaSpec’s proprietary detection technologies, AnaSpec FRET-based MMP substrates were recently used in a double differential in vivo zymography study at the University of New Brunswick.

In the study by Dr. Bryan Crawford, a mixture of QXL™ 570/5-TAMRA-labeled Peptide I incorporating a red fluorophore, and QXL™ 520/5-FAM-labeled Peptide XIII incorporating a green fluorophore were used to detect differential breakdown of the two different MMP substrates in zebrafish. Peptide I was broken down mostly in the maturing myotome boundaries, whereas Peptide XIII was broken down much more strongly in the notochord sheath.

Synthetic FRET peptide substrates provide a reliable method for monitoring enzyme activity by following substrate cleavage. Through the innovative work of researchers such as Dr. Crawford, the applications of AnaSpec’s FRET technologies continue to be expanded.
Company Info
AnaSpec, Inc. is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and combinatorial chemistry.