posted 12-08-2003 09:58 AM            

1) Is there new knowledge on this topic, a new powerful protocol? Otherwise I would like to assemble the gene from overlapping oligonucleotides covering both strands completely, maybe followed by PCR-amplification... any suggestions?

2) Is there a public domain software which helps to split down the DNA sequence to the level of oligonucleotides, helping in avoiding self-complementary oligonucleotides and checking for repeats or other homologous regions that could interfere with correct assembly of the gene?

Thanking you in advance - best regards
larry
E-mail:mantou@idvweb.com

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Dear Larry,

please take a look at
http://mcl1.ncifcrf.gov/dnaworks/dnaworks.html http://molbio.info.nih.gov/dnaworks/

DNAWorks automates the design of oligonucleotides for PCR-based gene synthesis.

Best

Frank S. Zollmann