home genetic news bioinformatics biotechnology literature journals ethics positions events sitemap


UBBFriend: Email This Page to Someone!
  HUM-MOLGEN
  Biotechnical requests and sources
  CpG rich southern probe

Post New Topic  Post A Reply
profile | register | preferences | faq | search

next newest topic | next oldest topic
Author Topic:   CpG rich southern probe
Cayli
Member
posted 05-31-2002 12:29 AM     Click Here to See the Profile for Cayli   Click Here to Email Cayli     Edit/Delete Message Reply w/Quote
I'm currently trying to probe a southern blot with a very CpG rich probe. To amplify the probe in the first palce I had to use PCR additive DMSO and betaine. I have tried adding these to my hyb buffer but have not had any luck.

Any suggestions would be much appreciated.

IP: 139.80.178.11

All times are ET (US)

next newest topic | next oldest topic

Administrative Options: Close Topic | Archive/Move | Delete Topic
Post New Topic  Post A Reply
Hop to:

Contact Us | HUM-MOLGEN

Copyright by HUM-MOLGEN 1995-2015

Powered by: Ultimate Bulletin Board, Version 5.44a
© Infopop Corporation (formerly Madrona Park, Inc.), 1998 - 2000.