posted 05-06-2002 08:31 PM            

Automated Plasmid Constructor

The Automated Plasmid Constructor (APC) for high throughput cloning

GeBA developed an "automated vector constructor" that can effectively combine several DNA fragments and ensure their correct orientation. Engineering of vectors is straightforward and easy using Orientation Enrichment Reaction (OER), a breakthrough automated technology in constructing plasmid for high throughput cloning. OER is GeBA patent pending reaction. In addition, a major benefit of this novel reaction is the ability to manipulate DNA sequences, to construct any kind of mutation (point mutation, deletions, insertions and others) as needed, in one single step within a very short time. All the process complete by the APC using the OER, the user provides only primers and DNA template. The first GeBA prototype capable to construct four different genetic constructs in just five hours.

The APC contains disposable parts to eliminate any cross-contamination (DNA templates, PCR products or PCR primers) during the process. Moreover, the APC serve as a "mini-laboratory" performing several independent processes, such as PCR, DNA fragment extraction from gels, gel analysis of the size and quantity of DNA or RNA fragments, and image processing. It is also possible to carry out two or more of these processes jointly and automatically. The APC harbor partly disposable detection and fragment extraction (DFE) unit that provides the biologic processing capacity, OER, to be fully automated; the aim of the unit is to detect and extract the precisely wanted DNA fragment from the separating matrix. The DFE unit consists of hardware and software that control the operating of the DFE unit; analyzing the gel images, detecting and extracting the desirable DNA fragment from the gel. The software runs under Windows.
1) GeBA presently provide Ultra Fast Plasmid Constructor or cloning services.

Please visit our website www.geba.org and contact Maurice Muller at miler-m@zahav.net.il for further information on GeBA's APC, International + (972) 53 732 431.