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Author Topic:   PCR amplification of genomic DNA isolated from amniotic fluid
chandakgrc
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posted 06-05-2001 01:29 PM     Click Here to See the Profile for chandakgrc   Click Here to Email chandakgrc     Edit/Delete Message Reply w/Quote
I am facing problems with PCR amplification of genomic DNA isolated from amniotic fluid. Can anybody share their experience and trouble shooting for the same. Is it due to some inhibitors or any other reason. Some such DNA samples do amplify after treatment with resins. However, I would like to know about a protocol for isolation of DNA from Amniotic fluid which does not lead to such problems

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IP: 164.100.12.112

David E. Kellogg
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posted 06-15-2001 12:57 PM     Click Here to See the Profile for David E. Kellogg   Click Here to Email David E. Kellogg     Edit/Delete Message Reply w/Quote
May be able to help troubleshoot. Have 11 years experience studying the minutiae of PCR and its kinetics. Protocols are dated, but might be useful.

IP: 195.110.144.210

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