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Author | Topic: DNA extraction from whole blood |
dchughes Member |
![]() ![]() ![]() ![]() ![]() Hi, I am planning to collect blood at a separate site for subsequent DNA extraction. I am after advice on the best methods of storage (type of tubes, temperature) and methods for DNA extraction (either in-house or commercial kits). Thanks Davdi ------------------ IP: 62.252.240.77 |
DSOUZA Moderator |
![]() ![]() ![]() ![]() ![]() Hi David: While I haven't isolated DNA from blood, I use a commercial kit from GENTRA [web-site: www.gentra.com] (distributor's address is given below) for my DNA isolations from fungi and yeast with good success. They also have protocols/technical reports for DNA isolation from blood. You can contact them at: Regards, Trevor D'Souza
quote: ------------------ IP: 35.8.243.18 |
daforerog Member |
![]() ![]() ![]() ![]() ![]() Hi, room temperature and vacutainer tubes with EDTA are very good for DNA isolation from human blood. Although DNA extraction kits work well (For example, DNA kits from Promega) one may prepare all the solutions by a 1 % of the cost of the kits, one good reference is Miller SA, Nucleic Acids Research, 1988, available free in Pub Med. Best wishes. Diego. IP: 168.176.160.13 |
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