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Author Topic:   DNA extraction from whole blood
dchughes
Member
posted 06-10-2000 11:08 AM     Click Here to See the Profile for dchughes   Click Here to Email dchughes     Edit/Delete Message Reply w/Quote
Hi,

I am planning to collect blood at a separate site for subsequent DNA extraction. I am after advice on the best methods of storage (type of tubes, temperature) and methods for DNA extraction (either in-house or commercial kits).
All comments appreciated.

Thanks

Davdi

------------------
David C. Hughes,
Reproductive Biology & Genetics Group,
The Wolfson Laboratories, 3rd Floor,
Dept. of Medicine,
University of Birmingham,
Birmingham
B15 2TT
UK

IP: 62.252.240.77

DSOUZA
Moderator
posted 06-13-2000 03:18 AM     Click Here to See the Profile for DSOUZA   Click Here to Email DSOUZA     Edit/Delete Message Reply w/Quote
Hi David:

While I haven't isolated DNA from blood, I use a commercial kit from GENTRA [web-site: www.gentra.com] (distributor's address is given below) for my DNA isolations from fungi and yeast with good success. They also have protocols/technical reports for DNA isolation from blood.

You can contact them at:
Philip Harris/FLOWGEN
Lynn Lane, Shenstone
Nr. Litchfield, Staffordshire WS14 OEE
Tel: (44) 1543 483054
Fax: (44) 1543 483055

Regards,

Trevor D'Souza


quote:
Originally posted by dchughes:
Hi,

I am planning to collect blood at a separate site for subsequent DNA extraction. I am after advice on the best methods of storage (type of tubes, temperature) and methods for DNA extraction (either in-house or commercial kits).
All comments appreciated.

Thanks

Davdi


------------------
Trevor M. D'Souza, Ph.D.
Research Associate
Dept. of Microbiology
Michigan State University
East Lansing, MI 48824.
USA

IP: 35.8.243.18

daforerog
Member
posted 06-05-2004 11:57 PM     Click Here to See the Profile for daforerog   Click Here to Email daforerog     Edit/Delete Message Reply w/Quote
Hi, room temperature and vacutainer tubes with EDTA are very good for DNA isolation from human blood. Although DNA extraction kits work well (For example, DNA kits from Promega) one may prepare all the solutions by a 1 % of the cost of the kits, one good reference is Miller SA, Nucleic Acids Research, 1988, available free in Pub Med.

Best wishes.

Diego.

IP: 168.176.160.13

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