home genetic news bioinformatics biotechnology literature journals ethics positions events sitemap


UBBFriend: Email This Page to Someone!
  HUM-MOLGEN
  Biotechnical requests and sources
  Gene synthesis experiment

Post New Topic  Post A Reply
profile | register | preferences | faq | search

next newest topic | next oldest topic
Author Topic:   Gene synthesis experiment
FS Zollmann
Administrator
posted 10-25-1999 12:26 PM     Click Here to See the Profile for FS Zollmann     Edit/Delete Message Reply w/Quote
Hi all,
I am planning a gene synthesis experiment.

QUESTIONS:

1) Is there new knowledge on this topic, a new powerful protocol? Otherwise I would like to assemble the gene from overlapping oligonucleotides covering both strands completely, maybe followed by PCR-amplification... any suggestions?

2) Is there a public domain software which helps to split down the DNA sequence to the level of oligonucleotides, helping in avoiding self-complementary oligonucleotides and checking for repeats or other homologous regions that could interfere with correct assembly of the gene?

Thanking you in advance - best regards
Ralf Koebnik
E-mail: koebnik@iname.com

IP: 160.45.191.21

All times are ET (US)

next newest topic | next oldest topic

Administrative Options: Close Topic | Archive/Move | Delete Topic
Post New Topic  Post A Reply
Hop to:

Contact Us | HUM-MOLGEN

Copyright by HUM-MOLGEN 1995-2015

Powered by: Ultimate Bulletin Board, Version 5.44a
© Infopop Corporation (formerly Madrona Park, Inc.), 1998 - 2000.