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Author Topic:   Gene synthesis experiment
FS Zollmann
posted 10-25-1999 12:26 PM     Click Here to See the Profile for FS Zollmann     Edit/Delete Message Reply w/Quote
Hi all,
I am planning a gene synthesis experiment.


1) Is there new knowledge on this topic, a new powerful protocol? Otherwise I would like to assemble the gene from overlapping oligonucleotides covering both strands completely, maybe followed by PCR-amplification... any suggestions?

2) Is there a public domain software which helps to split down the DNA sequence to the level of oligonucleotides, helping in avoiding self-complementary oligonucleotides and checking for repeats or other homologous regions that could interfere with correct assembly of the gene?

Thanking you in advance - best regards
Ralf Koebnik
E-mail: koebnik@iname.com


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