posted 05-08-2003 09:09 PM            

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I am interested in analyze the whole genes BRCA1 and BRCA2 for mutations in Mexican population. I will appreciatte information regarding the best way to do it, in order to simply the work and do it less expensive.
At the same time I am requesting some collaboration.

IP: 148.204.162.5

posted 01-04-2004 08:54 AM            

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deleted

[This message has been edited by imtiazshafiq (edited 08-07-2009).]

IP: 66.198.26.4

posted 03-25-2004 12:32 PM            

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Hello Dear,
As a large no. of BRCA1 & BRCA2 mutations are alredy known, you can use ASO-Hybridization for known mutations which is very sensitive and less expensive. In most of the families , known mutations will be found. For rest of familie you can go for PCR-SSCP. It wil let you know if any mutation is present in a patient or not. Then PCR products with migration in PAGE indicative of presence of mutations will be sequenced.
PLease let me know what type of collaboration do you want.
You can ring me at 0092-0303-6710865

Zafar Iqbal,
Clinical Molecular Genetics Lab.& Cancer Research Centre, National Institute for Biotechnology & Genetic Engineering (NIBGE),
Faisalabad, Pakistan.

IP: 202.59.89.36

posted 03-25-2004 12:42 PM            

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Hello Dear,
It is continous with first mail.
There are two ways we can help you
1) You invite our expert to come to your institute. In this way, You will have to fund him/her for his stay at leat for six months.
2) You can come to our Lab. You will have an advantage that living expenses are very less here (US$ 100/month). However, cost of consumables will be beared by you. Please note that we have all facilities an experties to help you.
It,s better to discuss it on Phone.
Zafar Iqbal,
Scientific Officer

IP: 202.59.89.36

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